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E.G.B.M. Bossink MSc (Elsbeth)

PhD Candidate

Expertise

Agriculture & Biology
Cell Culture
Endothelial Cells
Organ-On-A-Chip
Engineering & Materials Science
Heterografts
Lactic Acid
Tissue
Tissue Engineering
Medicine & Life Sciences
Poly(Lactide)

Research

MICRO-GUT project 

Organs-on-a-chip devices are miniaturized microfluidic devices containing human cells or grown 3D human tissue to recapitulate the in vivo tissue. These devices are claimed to be more accurate models than 2D cell studies, and furthermore they reduce the need for animal experiments.

In organ-on-chip devices, and specifically gut-on-a-chip devices, oxygen concentration is a tremendously important regulatory parameter. In the lumen of the human intestine, the commensal microbiome resides under anaerobic conditions, while a functional intestinal epithelium requires an aerobic environment. This results in a steep oxygen gradient along the radial axis of the intestine. This oxygen gradient is essential to be mimicked in gut-on-a-chip systems to be able to co-culture intestinal epithelial cells with the human microbiome. The oxygen gradient affects the organization, development and dynamic nature of the gut microbiome and the intestinal physiology. A gut-on-a-chip system in which this oxygen gradient can be controlled and monitored would allow to investigate the functioning of the human intestine as well as the effect of disturbances in the human microbiome to the intestinal epithelial cells. 

Publications

Recent
Komen, J. , Bossink, E. G. B. M. , van den Berg, A. , & van der Meer, A. (2021). Comparing on-chip colorectal cancer spheroid drug response to xenografts. Poster session presented at EUROoCS Conference 2021.
Bossink, E. G. B. M. , Vollertsen, A. R., Hagen , L. P. , van der Meer, A. , Segerink, L. , & Odijk, M. (2021). Multiplexed Organ-on-Chips with integrated macro valves for automated cell culture. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 (pp. 1385-1386)
Bossink, E. G. B. M. , Vollertsen, A. R. , Segerink, L. , van der Meer, A. , & Odijk, M. (2021). Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10-14 October 2021, Palm Springs, CA, USA (pp. 1269-1270)
Bossink, E. G. B. M. , Zakharova, M. , Odijk, M. , & Segerink, L. (2020). Measuring barrier function in a gut-on-chip. In μTAS 2020: The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 943-944)
Bossink, E. G. B. M. , Vollertsen, A. R. , Segerink, L. I. , & Odijk, M. (2019). Macro valve and peristaltic pump with cleanroom-free fabrication for multiplexed organ-on-chip applications. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (23 ed., pp. 1664-1665). The Chemical and Biological Microsystems Society.

Contact Details

Visiting Address

University of Twente
Faculty of Electrical Engineering, Mathematics and Computer Science
Carré (building no. 15), room C2433
Hallenweg 23
7522NH  Enschede
The Netherlands

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Mailing Address

University of Twente
Faculty of Electrical Engineering, Mathematics and Computer Science
Carré  C2433
P.O. Box 217
7500 AE Enschede
The Netherlands