Welcome...

dr. F.T.G. van den Brink (Floris)

Researcher

About Me

Education and projects

2008 – 2011: Master Electrical Engineering, University of Twente
Specialization: Microsystems and Microelectronics
Graduation topic: Lab on a Chip

2003 – 2008: Bachelor Electrical Engineering, University of Twente
Minor: Industrial Engineering & Management

List of projects

2011: Feasibility study
BIOS group and Hitachi High Technologies, Japan
Electrical cell lysis in blood for diagnostic equipment

2010: Master assignment
BIOS – Lab on a Chip group
Parallel sampling from individual cells on a microchip: towards a parallel single cell analysis platform

2009: Internship
Institute of Bioengineering and Nanotechnology, A*STAR, Singapore
Comparison of fluorescent DNA dyes for real-time gene synthesis

2008: Bachelor assignment
BIOS – Lab on a Chip group
Optical detection of the conversion efficiency of a microchip for medical applications

Expertise

Msh Release-Inhibiting Hormone
Mass Spectrometry
Electrochemical Cells
Mass Spectrometry
Proteins
Exercise Test
Microfluidics
Proteins

Research

Project: Miniaturization and integration of an electrochemical cell, a liquid chromatography column and a nano-electrospray ionization tip for on-line mass spectrometry on a disposable plastic microfluidic platform for drug screening and proteomics. 

For details see the Projects tab.

Publications

Recent Articles
van den Brink, F. T. G., Asthana, A., Bomer, J. G., Tolner, E. A., van den Maagdenberg, A. M. J. M., & Odijk, M. (2017). MINIATURIZED HYBRID PUSH-PULL PERFUSION PROBE FOR SAMPLING WITH HIGH SPATIAL AND TEMPORAL RESOLUTION. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (21 ed., pp. 215-216). (MicroTAS). Savannah: Chemical and Biological Microstystems Society.
van den Brink, F. T. G., Zhang, T., Ma, L., Odijk, M., Olthuis, W., Permentier, H. P., ... van den Berg, A. (2015). Electrochemical protein cleavage in a microfluidic cell with integrated boron doped diamond electrodes. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015) (pp. 1692-1694). (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS). Chemical and Biological Micro Systems Society.
van den Brink, F. T. G., Büter, L., Odijk, M., Olthuis, W., Karst, U., & van den Berg, A. (2013). Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle. In R. Zengerle (Ed.), 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS (pp. 11-13). (MicroTAS; No. MicroTAS 2013). Freiburg: The Printing House.

UT Research Information System

Projects

Electrochemistry – Liquid Chromatography – Mass Spectrometry for drug screening and proteomics

Introduction

In this project, the goal for BIOS develops a miniaturized electrochemical cell for on-line use with liquid chromatography and mass-spectrometry. Four different groups from four different fields of research work in a cooperative project funded by Dutch technology foundation STW (Stichting voor de Technische Wetenschappen). In (bio)analytical chemistry, pharmaceutics and proteomics, tools like liquid chromatography (LC) and mass spectrometry (MS) are frequently used. LC and MS are often combined with electrochemical cells used for different kinds of electrochemical conversions, which is also the common denominator in this project.

In mass spectrometry only charged species can be detected. Therefore, electrochemical conversions are used (among other methods) in (bio)analytical chemistry to provide charge to otherwise neutral molecules. The method of choice for drug metabolites and peptides is electrospray ionization (ESI), in which the fragments to be analyzed are charged using a high voltage spray needle.

Electrochemical oxidation of pharmaceuticals can be used to mimic the metabolic pathways occurring in the body. This is a way to accelerate in vitro tests for new potential drugs candidates.

Protein digestion and subsequent analysis of the resulting peptide mixture by mass spectrometry has become a powerful method for protein identification used especially in proteomics. Protein digestion or cleavage is done traditionally by enzymatic or chemical means. Important for the cleavage is the specific position at which the proteins are cleaved. Also, the cleavage must not produce unwanted, unspecific side products. Electrochemical cleavage might prove to be an attractive alternative.

Project goals

The BIOS – Lab on a Chip group is specialized in designing and fabricating microfluidic chips for

biomedical applications. In this project, the goal is to develop a miniaturized electrochemical cell with an integrated LC column and ESI needle for on-line use with MS. Miniaturization is beneficial, because of the increased surface to volume ratio. Therefore, high turnover rates for electrochemical metabolism and protein cleavage can be achieved. This integrated system will be produced in a plastic material with a fast fabrication process, to achieve a cheap disposable platform that will be used to obtain analytical results for pharmaceutical and proteomics applications.

Interested?

If you are interested and for instance would like to do your (master or bachelor) graduation work or a practical term, please contact me or my colleagues Mathieu Odijk or Wouter Olthuis.

Partners

Prof. dr. R. Bischoff
Rijksuniversiteit Groningen
Centre for Pharmacy & Analytical Biochemistry

Dr. A.P. Bruins
Rijksuniversiteit Groningen
Centre for Pharmacy & Mass Spectrometry Core Facility

Prof. dr. U. Karst
Universität Münster
Institute of Inorganic & Analytical Chemistry

Funding

Technologiestichting STW
Postbus 3021
3502 GA Utrecht
Nederland

Contact Details

Visiting Address

University of Twente
Faculty of Electrical Engineering, Mathematics & Computer Science
Carré (building no. 15), room 2431
Hallenweg 23
7522NH  Enschede
The Netherlands

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Mailing Address

University of Twente
Faculty of Electrical Engineering, Mathematics & Computer Science
Carré  2431
P.O. Box 217
7500 AE Enschede
The Netherlands