Education and projects
2008 – 2011: Master Electrical Engineering, University of Twente
Specialization: Microsystems and Microelectronics
Graduation topic: Lab on a Chip
2003 – 2008: Bachelor Electrical Engineering, University of Twente
Minor: Industrial Engineering & Management
List of projects
2011: Feasibility study
BIOS group and Hitachi High Technologies, Japan
Electrical cell lysis in blood for diagnostic equipment
2010: Master assignment
BIOS – Lab on a Chip group
Parallel sampling from individual cells on a microchip: towards a parallel single cell analysis platform
Institute of Bioengineering and Nanotechnology, A*STAR, Singapore
Comparison of fluorescent DNA dyes for real-time gene synthesis
2008: Bachelor assignment
BIOS – Lab on a Chip group
Optical detection of the conversion efficiency of a microchip for medical applications
Project: Miniaturization and integration of an electrochemical cell, a liquid chromatography column and a nano-electrospray ionization tip for on-line mass spectrometry on a disposable plastic microfluidic platform for drug screening and proteomics.
For details see the Projects tab.
UT Research Information System
Electrochemistry – Liquid Chromatography – Mass Spectrometry for drug screening and proteomics
In this project, the goal for BIOS develops a miniaturized electrochemical cell for on-line use with liquid chromatography and mass-spectrometry. Four different groups from four different fields of research work in a cooperative project funded by Dutch technology foundation STW (Stichting voor de Technische Wetenschappen). In (bio)analytical chemistry, pharmaceutics and proteomics, tools like liquid chromatography (LC) and mass spectrometry (MS) are frequently used. LC and MS are often combined with electrochemical cells used for different kinds of electrochemical conversions, which is also the common denominator in this project.
In mass spectrometry only charged species can be detected. Therefore, electrochemical conversions are used (among other methods) in (bio)analytical chemistry to provide charge to otherwise neutral molecules. The method of choice for drug metabolites and peptides is electrospray ionization (ESI), in which the fragments to be analyzed are charged using a high voltage spray needle.
Electrochemical oxidation of pharmaceuticals can be used to mimic the metabolic pathways occurring in the body. This is a way to accelerate in vitro tests for new potential drugs candidates.
Protein digestion and subsequent analysis of the resulting peptide mixture by mass spectrometry has become a powerful method for protein identification used especially in proteomics. Protein digestion or cleavage is done traditionally by enzymatic or chemical means. Important for the cleavage is the specific position at which the proteins are cleaved. Also, the cleavage must not produce unwanted, unspecific side products. Electrochemical cleavage might prove to be an attractive alternative.
The BIOS – Lab on a Chip group is specialized in designing and fabricating microfluidic chips for
biomedical applications. In this project, the goal is to develop a miniaturized electrochemical cell with an integrated LC column and ESI needle for on-line use with MS. Miniaturization is beneficial, because of the increased surface to volume ratio. Therefore, high turnover rates for electrochemical metabolism and protein cleavage can be achieved. This integrated system will be produced in a plastic material with a fast fabrication process, to achieve a cheap disposable platform that will be used to obtain analytical results for pharmaceutical and proteomics applications.
Prof. dr. R. Bischoff
Centre for Pharmacy & Analytical Biochemistry
Dr. A.P. Bruins
Centre for Pharmacy & Mass Spectrometry Core Facility
Prof. dr. U. Karst
Institute of Inorganic & Analytical Chemistry
3502 GA Utrecht