dr. G.M.J. Segers - Nolten (Ine) | University of Twente

In 1974 Ine started her HBO education ‘Hoger Natuur Wetenschappelijk Onderwijs (HNWO)’ at the Analistenschool in Groningen. She graduated in 1978 and started working as a biochemical research assistant at the Afdeling Nucleaire Geneeskunde, Academisch Ziekenhuis Groningen on a project concerning hormone dependence of breast tumor development. In 1981 she moved to the University of Twente, Technische Hogeschool Twente at that time, where she was appointed as one of the first members of the starting Biophysical Engineering group headed by Professor Jan Greve. Here she was responsible for setting up a biochemical/cell biological laboratory. She continued working as a research assistant within this group, and participated in many different projects. From 1998 till 2003 she worked as a PhD student within the BPE group on a project using single molecule fluorescence to study the human nucleotide excision DNA repair system. After obtaining her doctoral degree she stayed as a research scientist in the BPE group, later the Nanobiophysics group. Currently she is working within the NBP alpha-synuclein/ Parkinson’s disease related theme and she is managing the MESA+ BioNanoLab at the University of Twente, as the head of the facility.

Medicine & Life Sciences

# Alpha-Synuclein
# Amyloid
# Amyloid Beta-Protein (40-42)
# Synucleins
# Thioflavin T

Chemistry

# Amyloid Fibril
# Protein

Engineering & Materials Science

# Molecules

MESA+ BioNanoLab (bionanolab)

Semerdzhiev, S. A. , Segers-Nolten, I., van der Schoot, P. , Blum, C. , & Claessens, M. M. A. E. (2023). SARS-CoV-2 N-protein induces the formation of composite α-synuclein/N-protein fibrils that transform into a strain of α-synuclein fibrils. Nanoscale, 15, 18337-18346. Advance online publication. https://doi.org/10.1039/d3nr03556e

Vaneyck, J., Yousif, T. A. , Segers-Nolten, I. , Blum, C. , & Claessens, M. M. A. E. (2023). Quantitative Seed Amplification Assay: A Proof-of-Principle Study. The Journal of physical chemistry B, 127(8), 1735-1743. Advance online publication. https://doi.org/10.1021/acs.jpcb.2c08326

Semerdzhiev, S. A. , Fakhree, M. A. A. , Segers-Nolten, I. , Blum, C. , & Claessens, M. M. A. E. (2022). Interactions between SARS-CoV-2 N-Protein and α-Synuclein Accelerate Amyloid Formation. ACS chemical neuroscience, 13(1), 143-150. https://doi.org/10.1021/acschemneuro.1c00666

Molenaar, R. , Chatterjee, S., Kamphuis, B. , Segers-Nolten, I. M. J. , Claessens, M. M. A. E. , & Blum, C. (2021). Nanoplastic sizes and numbers: Quantification by single particle tracking. Environmental Science: Nano, 8(3), 723-730. https://doi.org/10.1039/d0en00951b

Vaneyck, J. , Segers-Nolten, I. , Broersen, K. , & Claessens, M. M. A. E. (2021). Cross-seeding of alpha-synuclein aggregation by amyloid fibrils of food proteins. Journal of biological chemistry, 296, Article 100358. https://doi.org/10.1016/J.JBC.2021.100358

Marczynski, M., Rickert, C. A. , Semerdzhiev, S. A., Van Dijk, W. R. , Segers-Nolten, I. M. J. , Claessens, M. M. A. E., & Lieleg, O. (2019). α-Synuclein Penetrates Mucin Hydrogels despite Its Mucoadhesive Properties. Biomacromolecules, 20(12), 4332-4344. https://doi.org/10.1021/acs.biomac.9b00905

Sidhu, A. , Vaneyck, J. , Blum, C. , Segers-Nolten, I. , & Subramaniam, V. (2018). Polymorph-specific distribution of binding sites determines thioflavin-T fluorescence intensity in α-synuclein fibrils. Amyloid, 25(3), 189-196. https://doi.org/10.1080/13506129.2018.1517736

Hassink, G. C. , Raiss, C. C. , Segers-Nolten, I. M. J. , van Wezel, R. J. A. , Subramaniam, V. , le Feber, J. , & Claessens, M. M. A. E. (2018). Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons. PLoS ONE, 13(3), Article e0193763. https://doi.org/10.1371/journal.pone.0193763

Fakhree, M. A. A. , Nolten, I. S. , Blum, C. , & Claessens, M. M. A. E. (2018). Different Conformational Subensembles of the Intrinsically Disordered Protein α-Synuclein in Cells. The journal of physical chemistry letters, 9(6), 1249-1253. https://doi.org/10.1021/acs.jpclett.8b00092

Book contributions:

M.R. Loken, C.I. Civin, V.O. Shah, M.J. Fackler, G.M.J. Segers-Nolten and L.W.M.M. Terstappen, (1992) Characterization of erythroid, lymphoid and monomyeloid lineages in normal human bone marrow. Flow Cytometry in Hematology, Eds. Laerum, O.D. and Bjerknes, R. Academic Press, Inc., 31-42

I. Segers-Nolten, M. van Raaij and V. Subramaniam, (2011) Biophysical Analysis of Amyloid Formation. Comprehensive Biomaterials, Eds.Ducheyne, P.,Healy, K., Hutmacher , D.W.,Grainger, D.W. and Kirkpatrick, C.J. Elsevier Ltd., Volume 2, 347-359