About Me
Development of a microfluidic device to enable the detection of cancer in urine samples of humans. My research focus is about surface functionalization with biorecognition elements to enable the extraction of cancerous biomarkers.
Expertise
Engineering & Materials Science
# Cell Culture
# Cells
# Dna
# Microfluidics
# Pharmacokinetics
Chemistry
# Concentration Profile
# Inhibition Of Growth
# Thiol
Publications
Recent
Kolkman, R. W. (2022).
Enrichment of hypermethylated DNA on chip for cancer diagnostics. [PhD Thesis - Research UT, graduation UT, University of Twente]. University of Twente.
https://doi.org/10.3990/1.9789036554855
Kolkman, R. W.
, Segerink, L.
, & Huskens, J. (2022).
Selective Enrichment of Hypermethylated DNA by a Multivalent Binding Platform.
Advanced materials interfaces,
9(35), [2201557].
https://doi.org/10.1002/admi.202201557
Kolkman, R. W., Michel-Souzy, S.
, Wasserberg, D.
, Segerink, L. I.
, & Huskens, J. (2022).
Density Control over MBD2 Receptor-Coated Surfaces Provides Superselective Binding of Hypermethylated DNA.
ACS applied materials & interfaces,
14(36), 40579-40589.
https://doi.org/10.1021/acsami.2c09641
van Dongen, J. E.
, Kolkman, R. W.
, Wasserberg, D.
, Knippenborg, L. V.
, Eijkel, J. C. T.
, & Segerink, L. (2021).
Localized surface plasmon resonance based gold nanoparticle-hairpin assay for optical detection of single DNA molecules. Poster session presented at 31st Anniversary World Congress on Biosensors, Biosensors 2021, Online.
Movilli, J.
, Kolkman, R. W., Rozzi, A., Corradini, R.
, Segerink, L. I.
, & Huskens, J. (2020).
Increasing the Sensitivity of Electrochemical DNA Detection by a Micropillar-Structured Biosensing Surface.
Langmuir : the ACS journal of surfaces and colloids,
36(16), 4272-4279.
https://doi.org/10.1021/acs.langmuir.0c00144
Komen, J., Westerbeek, E. Y.
, Kolkman, R. W., Roesthuis, J.
, Lievens, C.
, van den Berg, A.
, & van der Meer, A. D. (2020).
Controlled pharmacokinetic anti-cancer drug concentration profiles lead to growth inhibition of colorectal cancer cells in a microfluidic device.
Lab on a chip,
20(17), 3167-3178.
https://doi.org/10.1039/d0lc00419g
van Dongen, J. E.
, Le The, H.
, Kolkman, R. W.
, Wasserberg, D.
, Huskens, J.
, Eijkel, J. C. T.
, & Segerink, L. (2019).
Gold Nanoparticle Assays: A New Golden Standard for Optical DNA Detection. Poster session presented at 7th CHemistry As INnovating Science Conference, CHAINS 2019, Veldhoven, Netherlands.
Contact Details
Visiting Address
University of Twente
Drienerlolaan 5
7522 NB Enschede
The Netherlands
Mailing Address
University of Twente
P.O. Box 217
7500 AE Enschede
The Netherlands