dr.ir. A.M. Leferink (Anne)

Anne is a senior lecturer in the BSc and MSc program Technical Medicine (University of Twente) in modules including Cell Biology (theory and practical course), Biomaterials for clinical interventions (also module-coordinator) and in the mastercourse Clinical Biotechnology (also course coordinator). Furthermore, she is involved in the Minor ICR&TIST in which she teaches a micro-module on 'Practices in the natural Sciences'.

From August 2021 till December 2022, Anne was appointed 0.2 FTE as teaching fellow on Challenge Based Learning. This fellowship allowed her to gain experience and to conduct research on Challenge Based Learning methods in education.

Anne is embedded in the Applied Stem Cell Technologies group in the faculty of Science and Technology. She conducts research in the field of Organ-on-a-chip technology. She is involved in the national LymphChip Consortium. and is a member of the Organ On a chip Centre Twente (OoCCT), the dutch Human Organ And Disease Model Technologies (hDMT) consortium and the European Organ on a chip Society (EUROoCS).

Moreover, Anne has a general interest in the development of biomedical devices and tools for medical interventions and diagnostics for both in vivo and extracorporeal applications. She works with several clinical and industrial partners in setting up clinical trials and optimizing designs of medical devices. She has gained expertise in a broad range of biomedical related topics ranging from the molecular cell biology level up to the functional level of the musculoskeletal system at the macro-scale.

Engineering & Materials Science

# Blood-Brain Barrier
# Bone
# Membranes
# Scaffolds
# Tissue
# Tissue Engineering

Medicine & Life Sciences

# Mesenchymal Stem Cells
# Tissue Engineering

Faculty of Science and Technology (TNW), Applied Stem Cell Technologies (AST)

Lymph-on-a-chip consortium (NWA)
Development and testing of medical devices for per- and post-operative treatment.

Educational Research:

The role of the 'teacher' in Challenge-Based Learning
Peer-Feedback and Peer-Assessment in bachelor courses
From 'push-to-pull': how to increase students' autonomy on learning.

Paul, A., van Dorp, T. , Leferink, A. M. , Vollertsen, A. R. , van der Meer, A. , & Odijk, M. (2023). 3D Porous Microfluidic Architectures for Heart-on-Chip. Poster session presented at 27th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2023, Katowice, Poland.

Conceição, F., Sousa, D. M. , Loessberg-Zahl, J. , Vollertsen, A. R., Neto, E., Søe, K., Paredes, J. , Leferink, A., & Lamghari, M. (2022). A metastasis-on-a-chip approach to explore the sympathetic modulation of breast cancer bone metastasis. Materials Today Bio, 13, Article 100219. https://doi.org/10.1016/j.mtbio.2022.100219

Zakharova, M. , Tibbe, M. P. , Koch, L. S. , Le-The, H. , Leferink, A. M. , van den Berg, A. , van der Meer, A. D. , Broersen, K. , & Segerink, L. I. (2021). Transwell-Integrated 2 µm Thick Transparent Polydimethylsiloxane Membranes with Controlled Pore Sizes and Distribution to Model the Blood-Brain Barrier. Advanced Materials Technologies, 6(12), Article 2100138. Advance online publication. https://doi.org/10.1002/admt.202100138

Schilder, W. H. , Tanumihardja, E. , Leferink, A. M. , van den Berg, A. , & Olthuis, W. (2020). Determining the antioxidant properties of various beverages using staircase voltammetry. Heliyon, 6(6), Article e04210. https://doi.org/10.1016/j.heliyon.2020.e04210

Tibbe, M. P. (2020). Improving Organ on‐Chip Physiology: Exploring the barrier in blood‐brain barrier on‐chip systems. [PhD Thesis - Research UT, graduation UT]. University of Twente. https://doi.org/10.3990/1.9789036549301

Avgidis, F. , Tibbe, M. , Leferink, A. , & Segerink, L. (2019). Assessing barrier properties using impedance spectroscopy in a semi-circular, blood-brain barrier on-chip. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 422-423). (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019). The Chemical and Biological Microsystems Society.

Avgidis, F. , Tibbe, M. P. , Leferink, A. M. , & Segerink, L. (2019). Asessing barrier properties using impedance spectroscopy in a semi-circular, blood-brain barrier on-chip. In Microtas 2019

Leferink, A. M. , Tibbe, M. P., Bossink, E. G. B. M. , de Heus, L. E. , van Vossen, H. , van den Berg, A. , Moroni, L. , & Truckenmüller, R. K. (2019). Shape-defined solid micro-objects from poly(D,L-lactic acid) as cell-supportive counterparts in bottom-up tissue engineering. Materials Today Bio, 4, Article 100025. https://doi.org/10.1016/j.mtbio.2019.100025

de Pinho, A. R. G., Odila, I. , Leferink, A. , van Blitterswijk, C., Camarero-Espinosa, S. , & Moroni, L. (2019). Hybrid polyester-hydrogel electrospun scaffolds for tissue engineering applications. Frontiers in bioengineering and biotechnology, 7(SEP), Article 231. https://doi.org/10.3389/fbioe.2019.00231

Leferink, A. M. , Reis, D. S. , van Blitterswijk, C. A. , & Moroni, L. (2018). An antibody based approach for multi-coloring osteogenic and chondrogenic proteins in tissue engineered constructs. Biomedical Materials (Bristol), 13(4), Article 044102. https://doi.org/10.1088/1748-605X/aab51e

Minol, J. P., Reinsch, I., Luik, M. , Leferink, A., Barth, M., Assmann, A., Lichtenberg, A., & Akhyari, P. (2018). Reaktive Sauerstoffspezies und Gefäßdegeneration. Zeitschrift fur Herz-, Thorax- und Gefasschirurgie, 32(3), 242-247. https://doi.org/10.1007/s00398-018-0227-9

Tibbe, M. P. , Le The, H. , Leferink, A. M. , Eijkel, J. C. T. , & Segerink, L. I. (2018). Porous sbum-micrometer PDMS membranes as cell culture substrates in a microfludiic chip. Poster session presented at 9th International Conference on Microtechnologies in Medicine and Biology, MMB 2018, Monterey, California, United States.

Tibbe, M. , Le The, H. , Eijkel, J. , van den Berg, A. , Leferink, A. , & Segerink, L. (2018). Transwell integrated porous sub-micrometer PDMS membranes as alternative cell culture platform. Poster session presented at EMBS Micro and Nantechnology in Medicine Conference, MNMC 2018, Kauai, Hawaii, United States.

Le-The, H. , Tibbe, M. , Loessberg-Zahl, J., Palma Do Carmo, M. , van der Helm, M. , Bomer, J. , Van Den Berg, A. , Leferink, A. , Segerink, L. , & Eijkel, J. (2018). Large-scale fabrication of free-standing and sub-μm PDMS through-hole membranes. Nanoscale, 10(16), 7711-7718. https://doi.org/10.1039/c7nr09658e

Koenrades, M. A. , Klein, A. , Leferink, A. M. , Slump, C. H. , & Geelkerken, R. H. (2018). Evolution of the Proximal Sealing Rings of the Anaconda Stent-Graft After Endovascular Aneurysm Repair. Journal of Endovascular Therapy, 25(4), 480-491. https://doi.org/10.1177/1526602818773085

Tibbe, M. P. , Leferink, A. M. , van den Berg, A. , Eijkel, J. C. T. , & Segerink, L. (2018). Microfluidic Gel Patterning Method by Use of a Temporary Membrane for Organ-On-Chip Applications. Advanced Materials Technologies, 3(3), Article 1700200. Advance online publication. https://doi.org/10.1002/admt.201700200

Peer-reviewer for several journals including: Biomaterials, Biofabrication, Advanced Technologies, Journal of Tissue Engineering and Regenerative Medicine, Integrative Biology and Acta Biomaterialia.

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Module or course coordinator:

MOD11 TM "Biomaterials"
MSc. course TM: Clinical Biotechnology
Orthopedics Workshop for Master TM

Lecturer:

MOD01 TM "Bouwstenen van de Mens" Introduction to Cell Biology and Project
MOD02 TM "Zicht op ziekte" Project Assessor
MOD11 TM "Biomaterials" Biomaterials and Project
MSc. course TM: Clinical Biotechnology
Micro-module lecturer in ICR&TIST minor

Other:

Personal Pursuit supervisor at ATLAS (University College Twente)
Internship supervisor BME and TM

Bachelor

Master

Courses in the current academic year are added at the moment they are finalised in the Osiris system. Therefore it is possible that the list is not yet complete for the whole academic year.

Tumor-on-a-chip models to study secretion factors involved in cancer metastasis (in collaboration with i3S, Porto, Portugal)
Development of an oxygen delivery device to prevent anastomotic leakage after esophageal tumor resection (in collaboration with ZGT, MST and UMCG)
Development of a Lymph-on-a-chip model (NWA consortium)

For education project, see 'education'

https://www.utoday.nl/news/69929/anne-leferink-wins-ut-education-award

https://fb.watch/9bko0Ap2hs/

https://www.ad.nl/enschede/ut-onderzoekster-anne-leferink-wint-knappe-koppen-prijs-viva400~ae530305/

+31534894445

a.m.leferink@utwente.nl