I love thinking that leads to understanding and action.
Expertise
Chemistry
- Procedure
- Optical Microscopy
- Phase Composition
Biochemistry, Genetics and Molecular Biology
- Sample
- Raman Spectroscopy
- Spectrum
- Molecule
Medicine and Dentistry
- Cells
Organisations
Research interest: Development of optical spectroscopy and microscopy techniques for applications in biophysics and biomedics.
A selection of highlights:
first to show Raman spectra of single cells (1990)
first to show Raman images of single cells (2003)
Realization and discovery of "easy CARS" (after A. Voroshilov) that enabled CARS microscopy (1995-1997)
Developed a suite of optical single molecule techniques (imaging, FCS, multicolor FCS, super-resolution localization) for application in biophysics (2000-2010)
Initiative of light-harvesting bacterial protein based biomimicry and associated technology (2002-2010)
Developed Raman integrated with scanning electron microscopy technology (1995-now)
Developed spontaneous and coherent Raman methods (entire career)
Founder of the company Hybriscan Technologies B.V. (2009)
Hybriscan commercialized Raman for SEM, an integrated approach (2011)
Hybriscan commercialized an automated Raman instrument for clinical applications (2022)
Publications
2025
Advances in Synovial Fluid Analysis for the Diagnosis of Crystal Arthropathies (2025)Arthritis care & research (E-pub ahead of print/First online). Niessink, T., Jansen, T. L., Janssen, M. & Otto, C.https://doi.org/10.1002/acr.25698Analytical solution of the classical Rayleigh length definition, including truncation at arbitrary values (2025)Journal of microscopy, 300(1), 124-133. Lenferink, A. & Otto, C.https://doi.org/10.1111/jmi.70016POS0375 characterization of calcium pyrophosphate crystals in synovial fluid with Raman spectroscopy reveals presence of triclinic CPP only (2025)Annals of the Rheumatic Diseases, 84(Suppl. 1), 620-621. Ionete, F. I., Niessink, T., Gerritsen, M., Ea, H. K., Richette, P., Welting, T., Pascart, T., Janssen, M., Otto, C. & Jansen, T.https://doi.org/10.1016/j.ard.2025.05.761An objective diagnosis of gout and calcium pyrophosphate deposition disease with machine learning of Raman spectra acquired in a point-of-care setting (2025)Rheumatology, 64(4), 1791-1798 . Niessink, T., Jansen, T. L., Coumans, F. A. W., Welting, T. J. M., Janssen, M. & Otto, C.https://doi.org/10.1093/rheumatology/keae472Raman Spectroscopy Reveals Microparticles in Synovial Fluids of Patients With Suspected Implant-Related Complications (2025)Journal of raman spectroscopy, 56(2), 127-134. Niessink, T., Schoenmakers, J. A., Janssen, M., Wouthuyzen-Bakker, M., Piersma, S., van Oosten, M., van Dijl, J., Otto, C. & Jansen, T.https://doi.org/10.1002/jrs.6753
2024
Identification of extracellular vesicles from their Raman spectra via self-supervised learning (2024)Scientific reports, 14(1). Article 6791. Jensen, M. N., Guerreiro, E. M., Enciso-Martinez, A., Kruglik, S. G., Otto, C., Snir, O., Ricaud, B. & Hellesø, O. G.https://doi.org/10.1038/s41598-024-56788-7Synovial fluid analysis with compensated polarization light microscopy: a physics approach to quantitative understanding of birefringence, polarization, and Maltese crosses (2024)Clinical rheumatology, 43(12), 3597-3602. Niessink, T., Jansen, T. L., Kleinherenbrink, M., Janssen, M. & Otto, C.https://doi.org/10.1007/s10067-024-07185-yA new perspective on crystallopathies in rheumatology and orthopaedics with Raman spectroscopy (2024)[Thesis › PhD Thesis - Research UT, graduation UT]. University of Twente. Niessink, T.https://doi.org/10.3990/1.9789036563475Discovery of calcite as a new pro-inflammatory calcium-containing crystal in human osteoarthritic synovial fluid (2024)Osteoarthritis and cartilage, 32(10), 1261-1272. Niessink, T., Stassen, R. H. M. J., Kischkel, B., Vuscan, P., Emans, P. J., van den Akker, G. G. H., Janssen, M., Joosten, L. A. B., Otto, C., Welting, T. J. M. & Jansen, T. L.https://doi.org/10.1016/j.joca.2024.05.004Diagnostic Accuracy of Raman Spectroscopy Integrated With Polarized Light Microscopy for Calcium Pyrophosphate–Associated Arthritis (2024)Arthritis care & research, 76(9), 1333-1341. Niessink, T., Janssen, M., Giesen, T., Efdé, M. N., Comarniceanu, A. C., Otto, C. & Jansen, T. L.https://doi.org/10.1002/acr.25350High-Resolution Raman Imaging of >300 Patient-Derived Cells from Nine Different Leukemia Subtypes: A Global Clustering Approach (2024)Analytical chemistry, 96(23), 9468–9477. Vanna, R., Masella, A., Bazzarelli, M., Ronchi, P., Lenferink, A., Tresoldi, C., Morasso, C., Bedoni, M., Cerullo, G., Polli, D., Ciceri, F., De Poli, G., Bregonzio, M. & Otto, C.https://doi.org/10.1021/acs.analchem.4c00787AB0098 Analysis of suspected implant related complications with Raman spectroscopy reveals polymer and titanium dioxide microparticles in synovial fluid (2024)Annals of the Rheumatic Diseases, 83(Suppl. 1), 1282-1283. Niessink, T., Schoenmakers, J., Janssen, M., Otto, C. & Jansen, T.https://doi.org/10.1136/annrheumdis-2024-eular.2165AB0099 CHARACTERIZATION OF MENISCI, LIGAMENTS AND SYNOVIAL MEMBRANES IN PATIENTS WITH OSTEOARTHRITIS AND CALCIUM PYROPHOSPHATE DEPOSITION (CPPD) BY HISTOLOGY AND RAMAN SPECTROSCOPY (2024)Annals of the Rheumatic Diseases, 83(Suppl. 1), 1283. Filippou, G., Sirotti, S., Maroni, P., Lombardi, G., Niessink, T., Janssen, M., Otto, C., Jansen, T., Mangiavini, L., Rossi, N., Peretti, G. & Sarzi-Puttini, P.https://doi.org/10.1136/annrheumdis-2024-eular.3478POS0162 Superiority of PLM followed by Raman spectroscopy as a two-stepped analysis in synovial fluid crystal identification (2024)Annals of the Rheumatic Diseases, 83(Suppl. 1), 309-310. Niessink, T., Janssen, M., Otto, C. & Jansen, T.https://doi.org/10.1136/annrheumdis-2024-eular.2171POS0562 The diagnostic performance of raman spectroscopy for diagnosing CPPD with respect to the 2023 ACR/EULAR CPPD classification criteria (2024)Annals of the Rheumatic Diseases, 83(Suppl. 1), 566. Niessink, T., Janssen, M., Otto, C. & Jansen, T.https://doi.org/10.1136/annrheumdis-2024-eular.2159High-resolution Raman imaging of >300 patient-derived cells from nine different leukemia subtypes: A global clustering approach (2024)[Working paper › Preprint]. ChemRxiv. Vanna, R., Masella, A., Bazzarelli, M., Ronchi, P., Lenferink, A. T. M., Tresoldi, C., Morasso, C., Bedoni, M., Cerullo, G., Polli, D., Ciceri, F., De Poli, G., Bregonzio, M. & Otto, C.https://doi.org/10.26434/chemrxiv-2024-xpzhc-v2Supplementary data to "High-Resolution Raman Imaging of >300 Patient-Derived Cells from Nine Different Leukemia Subtypes: A Global Clustering Approach" (2024)[Dataset Types › Dataset]. Zenodo. Vanna, R., Masella, A., Manuela, B., Lenferink, A., Morasso, C., Cerullo, G., Polli, D., Bregonzio, M. & Otto, C.https://doi.org/10.5281/zenodo.11195903Raman spectroscopic analysis of joint capsule calcification of the fingers (2024)Clinical rheumatology, 43, 1783-1784. Jansen, T. L., Janssen, M., Otto, C., Vosters, J. L. G. & Niessink, T.https://doi.org/10.1007/s10067-024-06941-4631-Discovery of calcite as a new pro-inflammatory calcium-containing crystal in human osteoarthritic synovial fluid (2024)Osteoarthritis and cartilage, 32(Suppl. 1), S438. Stassen, R., Niessink, T., Akker, G. v. d., Emans, P., Otto, C., Welting, T. & Jansen, T.https://doi.org/10.1016/j.joca.2024.02.644High-resolution Raman imaging of >300 cells from human patients affected by nine different leukemia subtypes: A global clustering approach (2024)[Working paper › Preprint]. ChemRxiv. Vanna, R., Masella, A., Bazzarelli, M., Ronchi, P., Lenferink, A. T. M., Tresoldi, C., Morasso, C., Bedoni, M., Cerullo, G., Polli, D., Ciceri, F., Poli, G. D., Bregonzio, M. & Otto, C.https://doi.org/10.26434/chemrxiv-2024-xpzhcThe Prevalence of Titanium Dioxide Particles in Synovial Fluid Samples Drops after European Union Ban (2024)Gout, Urate, and Crystal Deposition Disease, 2(1), 45-51. Niessink, T., Janssen, M., Jansen, T. L. & Otto, C.https://doi.org/10.3390/gucdd2010004
2023
Test characteristics of Raman spectroscopy integrated with polarized light microscopy for the diagnosis of acute gouty arthritis (2023)Joint bone spine, 90(6). Article 105611. Niessink, T., Giesen, T., Efdé, M., Comarniceanu, A., Janssen, M., Otto, C. & Jansen, T. L.https://doi.org/10.1016/j.jbspin.2023.105611Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis (2023)Journal of Extracellular Vesicles, 12(11). Article 12376. Lozano-Andrés, E., Enciso-Martinez, A., Gijsbers, A., Ridolfi, A., Van Niel, G., Libregts, S. F. W. M., Pinheiro, C., van Herwijnen, M. J. C., Hendrix, A., Brucale, M., Valle, F., Peters, P. J., Otto, C., Arkesteijn, G. J. A. & Wauben, M. H. M.https://doi.org/10.1002/jev2.12376Clinical image: synovitis of the metacarpophalangeal joints (2023)Annals of the Rheumatic Diseases, 82, 992-993. Weele, L. v. d., Niessink, T., Maas, M., Griot, P. D., Janssen, M., Otto, C., Jansen, T. L., Tas, S. W. & Vries, N. d.https://doi.org/10.1136/ard-2022-223290Calcium carbonates are novel positive birefringent crystals in synovial fluid (2023)Clinical rheumatology, 42, 635-636. Niessink, T., Otto, C., Janssen, M. & Jansen, T. L.https://doi.org/10.1007/s10067-022-06441-3
Research profiles
I enjoy teaching
Affiliated study programs
Courses academic year 2025/2026
Courses in the current academic year are added at the moment they are finalised in the Osiris system. Therefore it is possible that the list is not yet complete for the whole academic year.
Courses academic year 2024/2025
Raman-based diagnostics
Biomolecular distributions in cells
Enzyme activity in cells with Resonance Raman spectroscopy and imaging
Address
University of Twente
Carré (building no. 15), room C4427
Hallenweg 23
7522 NH Enschede
Netherlands
University of Twente
Carré C4427
P.O. Box 217
7500 AE Enschede
Netherlands
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