Welcome...

dr. C. Otto (Cees)

Associate Professor

About Me

I love thinking that leads to understanding and action.


Expertise

Medicine & Life Sciences
Extracellular Vesicles
Raman Spectrum Analysis
Engineering & Materials Science
Gold Nanorods
Lipids
Microscopic Examination
Raman Scattering
Raman Spectroscopy
Chemistry
Coherent Anti-Stokes Resonance Raman Spectroscopy

Research

Research interest: Development of optical spectroscopy and microscopy techniques for applications in biophysics and biomedics. 

A selection of highlights:

first to show Raman spectra of single cells (1990)

first to show Raman images of single cells (2003)

Realization and discovery of "easy CARS" (after A. Voroshilov)  that enabled CARS microscopy (1995-1997)

Developed a suite of optical single molecule techniques (imaging, FCS, multicolor FCS, super-resolution localization) for application in biophysics (2000-2010)

Initiative of light-harvesting bacterial protein based biomimicry and associated technology (2002-2010)

Developed Raman integrated with scanning electron microscopy technology (1995-now)

Developed spontaneous and coherent Raman methods (entire career)

Founder of the company Hybriscan Technologies B.V. (2009)

Hybriscan commercialized Raman for SEM, an integrated approach (2011)

Hybriscan commercialized an automated Raman instrument for clinical applications (2022)

Publications

Recent
Venzac, B., Deng, S., Mahmoud, Z. , Lenferink, A., Costa, A., Bray, F. , Otto, C., Rolando, C. , & Le Gac, S. (2021). PDMS Curing Inhibition on 3D-Printed Molds: Why? Also, How to Avoid It? Analytical chemistry, 93(19), 7180-7187. https://doi.org/10.1021/acs.analchem.0c04944
de Wolf, J. R., Lenferink, A. , Lenferink, A. T. M. , Otto, C. , & Bosschaart, N. (2021). Changes in human milk lipid composition and conformational state during a single breastfeed. In E. Beaurepaire, A. Ben-Yakar, & Y. Park (Eds.), European Conferences on Biomedical Optics, ECBO 2021: Advances in Microscopic Imaging III [ETu2A.26] (SPIE Conference Proceedings; Vol. 11922). The Optical Society. https://doi.org/10.1117/12.2615815
Beekman, P. , Enciso-Martinez, A., Pujari, S., Zuilhof, H. , Terstappen, L. , Otto, C. , & Le Gac, S. (2020). Organosilicon interaction with biological membranes. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1035-1036). The Chemical and Biological Microsystems Society.
Venzac, B., Deng, S., Mahmoud, Z. , Lenferink, A., Bray, F. , Otto, C., Rolando, C. , & Le Gac, S. (2020). Why are 3D-printed molds inhibiting PDMS curing? In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 282-283). The Chemical and Biological Microsystems Society.
Beekman, P. , Enciso-Martinez, A., Piontek, M. , Terstappen, L., Roos, W. , Otto, C. , & Le Gac, S. (2020). Multi-modal analysis of tumor-derived extracellular vesicles immunocaptured from plasma. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 404-405). (MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences). The Chemical and Biological Microsystems Society.
Venzac, B. , le Gac, S. , Otto, C. , & Lenferink, A. T. M. (2019). PDMS curing inhibition by 3D-printed templates. Why? And how to avoid it. Poster session presented at NanoBioTech-Montreux 2019, Montreux, Switzerland.
Venzac, B. , le Gac, S., Yang, S. , Lenferink, A. T. M. , & Otto, C. (2019). PDMS curing inhibition by 3D-printed templates. Why? And how to avoid it. Poster session presented at 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, Basel, Switzerland.
Nair, S. , Otto, C. , Duits, M. , & Mugele, F. (2019). Microfluidics and Raman imaging as a platform to study low salinity enhanced oil recovery. Poster session presented at Physics@Veldhoven 2019, Veldhoven, Netherlands.

UT Research Information System

Education

I enjoy teaching

 

Affiliated Study Programmes

Bachelor

Master

Courses Academic Year  2022/2023

Courses in the current academic year are added at the moment they are finalised in the Osiris system. Therefore it is possible that the list is not yet complete for the whole academic year.
 

Courses Academic Year  2021/2022

Projects

Raman-based diagnostics

Biomolecular distributions in cells

Enzyme activity in cells with Resonance Raman spectroscopy and imaging

Contact Details

Visiting Address

University of Twente
Faculty of Science and Technology
Carré (building no. 15), room C4427
Hallenweg 23
7522NH  Enschede
The Netherlands

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Mailing Address

University of Twente
Faculty of Science and Technology
Carré  C4427
P.O. Box 217
7500 AE Enschede
The Netherlands