dr. C. Otto (Cees)

I love thinking that leads to understanding and action.

Medicine & Life Sciences

# Extracellular Vesicles
# Raman Spectrum Analysis

Engineering & Materials Science

# Gold Nanorods
# Lipids
# Microscopic Examination
# Raman Scattering
# Raman Spectroscopy

Chemistry

# Coherent Anti-Stokes Resonance Raman Spectroscopy

Faculty of Science and Technology (TNW), Medical Cell Biophysics (MCBP)

Research interest: Development of optical spectroscopy and microscopy techniques for applications in biophysics and biomedics.

A selection of highlights:

first to show Raman spectra of single cells (1990)

first to show Raman images of single cells (2003)

Realization and discovery of "easy CARS" (after A. Voroshilov) that enabled CARS microscopy (1995-1997)

Developed a suite of optical single molecule techniques (imaging, FCS, multicolor FCS, super-resolution localization) for application in biophysics (2000-2010)

Initiative of light-harvesting bacterial protein based biomimicry and associated technology (2002-2010)

Developed Raman integrated with scanning electron microscopy technology (1995-now)

Developed spontaneous and coherent Raman methods (entire career)

Founder of the company Hybriscan Technologies B.V. (2009)

Hybriscan commercialized Raman for SEM, an integrated approach (2011)

Hybriscan commercialized an automated Raman instrument for clinical applications (2022)

Niessink, T. , Otto, C., Janssen, M., & Jansen, T. L. (2023). Calcium carbonates are novel positive birefringent crystals in synovial fluid. Clinical rheumatology, 42, 635-636. https://doi.org/10.1007/s10067-022-06441-3

Niessink, T. , Kuipers, C., Jong, B. Z. D., Lenferink, A. T. M., Janssen, M., Jansen, T. L. , & Otto, C. (2023). Raman hyperspectral imaging detects novel and combinations of crystals in synovial fluids of patients with a swollen joint. Journal of raman spectroscopy, 54(1), 47-53. https://doi.org/10.1002/jrs.6452

Lozano-Andrés, E. , Enciso-Martinez, A., Gijsbers, A., Libregts, S. F. W. M., Pinheiro, C., Niel, G. V., Hendrix, A., Peters, P. J. , Otto, C., Arkesteijn, G. J. A., & Wauben, M. H. M. (2022). Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis. bioRxiv. https://doi.org/10.1101/2022.08.31.506022

Weele, L. V. D. , Niessink, T., Maas, M., Griot, P. D., Janssen, M. , Otto, C., Jansen, T. L., Tas, S. W., & Vries, N. D. (2022). Clinical image: synovitis of the metacarpophalangeal joints. Annals of the rheumatic diseases, [223290]. https://doi.org/10.1136/ard-2022-223290

Lak, N. S. M., van der Kooi, E. J. , Enciso-Martinez, A., Lozano-Andrés, E. , Otto, C., Wauben, M. H. M., & Tytgat, G. A. M. (2022). Extracellular Vesicles: A New Source of Biomarkers in Pediatric Solid Tumors? A Systematic Review. Frontiers in oncology, 12, [887210]. https://doi.org/10.3389/fonc.2022.887210

Niessink, T., Ringoot, J., Janssen, M. , Otto, C., & Jansen, T. L. T. A. (2022). Clinical Images: Detection of titanium dioxide particles by Raman spectroscopy in synovial fluid from a swollen ankle. Arthritis & rheumatology, 74(6), 1069-1069. https://doi.org/10.1002/art.42078

Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Costa, A., Bray, F. , Otto, C., Rolando, C. , & Le Gac, S. (2021). PDMS Curing Inhibition on 3D-Printed Molds: Why? Also, How to Avoid It? Analytical chemistry, 93(19), 7180-7187. https://doi.org/10.1021/acs.analchem.0c04944

Beekman, P. , Enciso-Martinez, A., Pujari, S. P. , Terstappen, L. W. M. M., Zuilhof, H. T. , Le Gac, S. , & Otto, C. (2021). Organosilicon uptake by biological membranes. Communications Biology, 4(1), [704]. https://doi.org/10.1038/s42003-021-02155-5

Beekman, P. , Enciso-Martinez, A., Pujari, S. P. , Terstappen, L. W. M. M., Zuilhof, H. , Le Gac, S. , & Otto, C. (2021). Author Correction: Organosilicon uptake by biological membranes. Communications Biology, 4(1), [812]. https://doi.org/10.1038/s42003-021-02338-0, https://doi.org/10.1038/s42003-021-02338-0

Beekman, P. , Enciso-Martinez, A., Pujari, S. P. , Terstappen, L. W. M. M., Zuilhof, H. , Le Gac, S. , & Otto, C. (2021). Publisher Correction: Organosilicon uptake by biological membranes. Communications Biology, 4(1), [813]. https://doi.org/10.1038/s42003-021-02344-2, https://doi.org/10.1038/s42003-021-02344-2

de Wolf, J. R., Lenferink, A., Lenferink, A. T. M. , Otto, C. , & Bosschaart, N. (2021). Changes in human milk lipid composition and conformational state during a single breastfeed. In E. Beaurepaire, A. Ben-Yakar, & Y. Park (Eds.), European Conferences on Biomedical Optics, ECBO 2021: Advances in Microscopic Imaging III [ETu2A.26] (SPIE Conference Proceedings; Vol. 11922). The Optical Society. https://doi.org/10.1117/12.2615815

Nair, S. (2021). Fast and non-invasive Raman imaging of material distributions at interfaces. [PhD Thesis - Research UT, graduation UT, University of Twente]. University of Twente. https://doi.org/10.3990/1.9789036553124

Nair, S., Gao, J. , Otto, C. , Duits, M. H. G. , & Mugele, F. (2021). Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy. Analytical chemistry, 93(38), 12966-12972. https://doi.org/10.1021/acs.analchem.1c02581

de Wolf, J. R., Lenferink, A., Lenferink, A. , Otto, C. , & Bosschaart, N. (2021). Evaluation of the changes in human milk lipid composition and conformational state with Raman spectroscopy during a breastfeed. Biomedical optics express, 12(7), 3934-3947. https://doi.org/10.1364/BOE.427646

Nair, S. , Gao, J. , Otto, C. , Duits, M. H. G. , & Mugele, F. (2021). In-situ observation of reactive wettability alteration using algorithm-improved confocal Raman microscopy. Journal of colloid and interface science, 584, 551-560. https://doi.org/10.1016/j.jcis.2020.10.016

Beekman, P. , Enciso-Martinez, A., Pujari, S., Zuilhof, H. , Terstappen, L. , Otto, C. , & Le Gac, S. (2020). Organosilicon interaction with biological membranes. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1035-1036). The Chemical and Biological Microsystems Society.

Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Bray, F. , Otto, C., Rolando, C. , & Le Gac, S. (2020). Why are 3D-printed molds inhibiting PDMS curing? In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 282-283). The Chemical and Biological Microsystems Society.

Beekman, P. , Enciso-Martinez, A., Piontek, M. , Terstappen, L., Roos, W. , Otto, C. , & Le Gac, S. (2020). Multi-modal analysis of tumor-derived extracellular vesicles immunocaptured from plasma. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 404-405). (MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences). The Chemical and Biological Microsystems Society.

Enciso-Martinez, A., Van Der Pol, E., Hau, C. M., Nieuwland, R. , Van Leeuwen, T. G. , Terstappen, L. W. M. M. , & Otto, C. (2020). Label-free identification and chemical characterisation of single extracellular vesicles and lipoproteins by synchronous Rayleigh and Raman scattering. Journal of Extracellular Vesicles, 9(1), [1730134]. https://doi.org/10.1080/20013078.2020.1730134

Rikkert, L. G. , Beekman, P., Caro, J. , Coumans, F. A. W. , Enciso-Martinez, A., Jenster, G. , Le Gac, S. , Lee, W. , van Leeuwen, T. G., Loozen, G. B. , Nanou, A., Nieuwland, R. , Offerhaus, H. L. , Otto, C., Pegtel, D. M., Piontek, M. C., van der Pol, E. , de Rond, L., Roos, W. H. , ... Terstappen, L. W. M. M. (2020). Cancer-ID: Toward identification of cancer by tumor-derived extracellular vesicles in blood. Frontiers in oncology, 10, [608]. https://doi.org/10.3389/fonc.2020.00608

Enciso Martinez, A. (2020). EV trapping: Raman characterization of single tumor-derived extracellular vesicles. [PhD Thesis - Research UT, graduation UT, University of Twente]. University of Twente. https://doi.org/10.3990/1.9789036550192

Nair, S. , Gao, J. , Yao, Q. , Duits, M. H. G. , Otto, C. , & Mugele, F. (2020). Algorithm-improved high speed and non-invasive confocal Raman imaging of two-dimensional materials. National Science Review, 7(3), 620-628. https://doi.org/10.1093/nsr/nwz177

O'Neill, D. B., Prezgot, D., Ianoul, A. , Otto, C. , Mul, G. , & Huijser, A. (2020). Silver Nanocubes Coated in Ceria: Core/Shell Size Effects on Light-Induced Charge Transfer. ACS Applied Materials and Interfaces, 12(1), 1905-1912. https://doi.org/10.1021/acsami.9b18393

Enciso-Martinez, A., van der Pol, E., Lenferink, A. T. M. , Terstappen, L. W. M. M. , van Leeuwen, T. G. , & Otto, C. (2020). Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles. Nanomedicine : nanotechnology, biology and medicine, 24, [102109]. https://doi.org/10.1016/j.nano.2019.102109

Lee, W., Lenferink, A. T. M. , Otto, C. , & Offerhaus, H. L. (2020). Classifying Raman Spectra of Extracellular Vesicles based on Convolutional Neural Networks for Prostate Cancer Detection. Journal of raman spectroscopy, 51(2), 293-300. https://doi.org/10.1002/jrs.5770

See all research output of this employee

I enjoy teaching

Bachelor

Biomedische Technologie

Master

Biomedical Engineering

Courses in the current academic year are added at the moment they are finalised in the Osiris system. Therefore it is possible that the list is not yet complete for the whole academic year.