dr. C. Otto (Cees)

I love thinking that leads to understanding and action.

Medicine & Life Sciences

# Extracellular Vesicles
# Raman Spectrum Analysis

Engineering & Materials Science

# Gold Nanorods
# Lipids
# Microscopic Examination
# Raman Scattering
# Raman Spectroscopy

Chemistry

# Coherent Anti-Stokes Resonance Raman Spectroscopy

Faculty of Science and Technology (TNW), Medical Cell Biophysics (MCBP)

Research interest: Development of optical spectroscopy and microscopy techniques for applications in biophysics and biomedics.

A selection of highlights:

first to show Raman spectra of single cells (1990)

first to show Raman images of single cells (2003)

Realization and discovery of "easy CARS" (after A. Voroshilov) that enabled CARS microscopy (1995-1997)

Developed a suite of optical single molecule techniques (imaging, FCS, multicolor FCS, super-resolution localization) for application in biophysics (2000-2010)

Initiative of light-harvesting bacterial protein based biomimicry and associated technology (2002-2010)

Developed Raman integrated with scanning electron microscopy technology (1995-now)

Developed spontaneous and coherent Raman methods (entire career)

Founder of the company Hybriscan Technologies B.V. (2009)

Hybriscan commercialized Raman for SEM, an integrated approach (2011)

Hybriscan commercialized an automated Raman instrument for clinical applications (2022)

Lozano-Andrés, E. , Enciso-Martinez, A., Gijsbers, A., Ridolfi, A., Van Niel, G., Libregts, S. F. W. M., Pinheiro, C., van Herwijnen, M. J. C., Hendrix, A., Brucale, M., Valle, F. , Peters, P. J. , Otto, C., Arkesteijn, G. J. A., & Wauben, M. H. M. (2023). Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis. Journal of Extracellular Vesicles, 12(11), Article 12376. Advance online publication. https://doi.org/10.1002/jev2.12376

Niessink, T., Giesen, T., Efdé, M., Comarniceanu, A., Janssen, M. , Otto, C., & Jansen, T. L. (2023). Test characteristics of Raman spectroscopy integrated with polarized light microscopy for the diagnosis of acute gouty arthritis. Joint bone spine, 90(6), Article 105611. Advance online publication. https://doi.org/10.1016/j.jbspin.2023.105611

Niessink, T. , Otto, C., Janssen, M., & Jansen, T. L. (2023). Calcium carbonates are novel positive birefringent crystals in synovial fluid. Clinical rheumatology, 42, 635-636. Advance online publication. https://doi.org/10.1007/s10067-022-06441-3

Niessink, T. , Kuipers, C., Jong, B. Z. D., Lenferink, A. T. M., Janssen, M., Jansen, T. L. , & Otto, C. (2023). Raman hyperspectral imaging detects novel and combinations of crystals in synovial fluids of patients with a swollen joint. Journal of raman spectroscopy, 54(1), 47-53. Advance online publication. https://doi.org/10.1002/jrs.6452

Lozano-Andrés, E. , Enciso-Martinez, A., Gijsbers, A., Libregts, S. F. W. M., Pinheiro, C., Niel, G. V., Hendrix, A., Peters, P. J. , Otto, C., Arkesteijn, G. J. A., & Wauben, M. H. M. (2022). Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis. bioRxiv. https://doi.org/10.1101/2022.08.31.506022

Weele, L. V. D. , Niessink, T., Maas, M., Griot, P. D., Janssen, M. , Otto, C., Jansen, T. L., Tas, S. W., & Vries, N. D. (2022). Clinical image: synovitis of the metacarpophalangeal joints. Annals of the rheumatic diseases, Article 223290. Advance online publication. https://doi.org/10.1136/ard-2022-223290

Lak, N. S. M., van der Kooi, E. J. , Enciso-Martinez, A., Lozano-Andrés, E. , Otto, C., Wauben, M. H. M., & Tytgat, G. A. M. (2022). Extracellular Vesicles: A New Source of Biomarkers in Pediatric Solid Tumors? A Systematic Review. Frontiers in oncology, 12, Article 887210. https://doi.org/10.3389/fonc.2022.887210

Niessink, T., Ringoot, J., Janssen, M. , Otto, C., & Jansen, T. L. T. A. (2022). Clinical Images: Detection of titanium dioxide particles by Raman spectroscopy in synovial fluid from a swollen ankle. Arthritis & rheumatology, 74(6), 1069-1069. Advance online publication. https://doi.org/10.1002/art.42078

Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Costa, A., Bray, F. , Otto, C., Rolando, C. , & Le Gac, S. (2021). PDMS Curing Inhibition on 3D-Printed Molds: Why? Also, How to Avoid It? Analytical chemistry, 93(19), 7180-7187. https://doi.org/10.1021/acs.analchem.0c04944

Beekman, P. , Enciso-Martinez, A., Pujari, S. P. , Terstappen, L. W. M. M., Zuilhof, H. T. , Le Gac, S. , & Otto, C. (2021). Organosilicon uptake by biological membranes. Communications Biology, 4(1), Article 704. https://doi.org/10.1038/s42003-021-02155-5

Beekman, P. , Enciso-Martinez, A., Pujari, S. P. , Terstappen, L. W. M. M., Zuilhof, H. , Le Gac, S. , & Otto, C. (2021). Author Correction: Organosilicon uptake by biological membranes. Communications Biology, 4(1), Article 812. https://doi.org/10.1038/s42003-021-02338-0, https://doi.org/10.1038/s42003-021-02338-0

Beekman, P. , Enciso-Martinez, A., Pujari, S. P. , Terstappen, L. W. M. M., Zuilhof, H. , Le Gac, S. , & Otto, C. (2021). Publisher Correction: Organosilicon uptake by biological membranes. Communications Biology, 4(1), Article 813. https://doi.org/10.1038/s42003-021-02344-2, https://doi.org/10.1038/s42003-021-02344-2

de Wolf, J. R., Lenferink, A., Lenferink, A. T. M. , Otto, C. , & Bosschaart, N. (2021). Changes in human milk lipid composition and conformational state during a single breastfeed. In E. Beaurepaire, A. Ben-Yakar, & Y. Park (Eds.), European Conferences on Biomedical Optics, ECBO 2021: Advances in Microscopic Imaging III Article ETu2A.26 (SPIE Conference Proceedings; Vol. 11922). The Optical Society. https://doi.org/10.1117/12.2615815

Nair, S. (2021). Fast and non-invasive Raman imaging of material distributions at interfaces. [PhD Thesis - Research UT, graduation UT, University of Twente]. University of Twente. https://doi.org/10.3990/1.9789036553124

Nair, S., Gao, J. , Otto, C. , Duits, M. H. G. , & Mugele, F. (2021). Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy. Analytical chemistry, 93(38), 12966-12972. https://doi.org/10.1021/acs.analchem.1c02581

de Wolf, J. R., Lenferink, A., Lenferink, A. , Otto, C. , & Bosschaart, N. (2021). Evaluation of the changes in human milk lipid composition and conformational state with Raman spectroscopy during a breastfeed. Biomedical optics express, 12(7), 3934-3947. https://doi.org/10.1364/BOE.427646

Nair, S. , Gao, J. , Otto, C. , Duits, M. H. G. , & Mugele, F. (2021). In-situ observation of reactive wettability alteration using algorithm-improved confocal Raman microscopy. Journal of colloid and interface science, 584, 551-560. https://doi.org/10.1016/j.jcis.2020.10.016

Beekman, P. , Enciso-Martinez, A., Pujari, S., Zuilhof, H. , Terstappen, L. , Otto, C. , & Le Gac, S. (2020). Organosilicon interaction with biological membranes. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1035-1036). The Chemical and Biological Microsystems Society.

Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Bray, F. , Otto, C., Rolando, C. , & Le Gac, S. (2020). Why are 3D-printed molds inhibiting PDMS curing? In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 282-283). The Chemical and Biological Microsystems Society.

Beekman, P. , Enciso-Martinez, A., Piontek, M. , Terstappen, L., Roos, W. , Otto, C. , & Le Gac, S. (2020). Multi-modal analysis of tumor-derived extracellular vesicles immunocaptured from plasma. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 404-405). (MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences). The Chemical and Biological Microsystems Society.