Carré
Guest staff

prof.dr. C. Otto (Cees)

Professor
TNW-BIS-MCBP
Carré C4427
c.otto@utwente.nl
+31534893159
Business card

I love thinking that leads to understanding and action.

Expertise

  • Chemistry

    • Procedure
    • Optical Microscopy

  • Biochemistry, Genetics and Molecular Biology

    • Sample
    • Raman Spectroscopy
    • Spectrum
    • Molecule

  • Medicine and Dentistry

    • Raman Spectrometry
    • Cells

Organisations

Research interest: Development of optical spectroscopy and microscopy techniques for applications in biophysics and biomedics. 

A selection of highlights:

first to show Raman spectra of single cells (1990)

first to show Raman images of single cells (2003)

Realization and discovery of "easy CARS" (after A. Voroshilov)  that enabled CARS microscopy (1995-1997)

Developed a suite of optical single molecule techniques (imaging, FCS, multicolor FCS, super-resolution localization) for application in biophysics (2000-2010)

Initiative of light-harvesting bacterial protein based biomimicry and associated technology (2002-2010)

Developed Raman integrated with scanning electron microscopy technology (1995-now)

Developed spontaneous and coherent Raman methods (entire career)

Founder of the company Hybriscan Technologies B.V. (2009)

Hybriscan commercialized Raman for SEM, an integrated approach (2011)

Hybriscan commercialized an automated Raman instrument for clinical applications (2022)

Publications

Jump to: 2024 | 2023 | 2022 | 2021 | 2020

2024

Diagnostic Accuracy of Raman Spectroscopy Integrated With Polarized Light Microscopy for Calcium Pyrophosphate–Associated Arthritis (2024)Arthritis care & research, 76(9), 1333-1341. Niessink, T., Janssen, M., Giesen, T., Efdé, M. N., Comarniceanu, A. C., Otto, C. & Jansen, T. L.https://doi.org/10.1002/acr.25350High-Resolution Raman Imaging of >300 Patient-Derived Cells from Nine Different Leukemia Subtypes: A Global Clustering Approach (2024)Analytical chemistry, 96(23), 9468–9477. Vanna, R., Masella, A., Bazzarelli, M., Ronchi, P., Lenferink, A., Tresoldi, C., Morasso, C., Bedoni, M., Cerullo, G., Polli, D., Ciceri, F., De Poli, G., Bregonzio, M. & Otto, C.https://doi.org/10.1021/acs.analchem.4c00787Supplementary data to "High-Resolution Raman Imaging of >300 Patient-Derived Cells from Nine Different Leukemia Subtypes: A Global Clustering Approach" (2024)[Dataset Types › Dataset]. Zenodo. Vanna, R., Masella, A., Manuela, B., Lenferink, A., Morasso, C., Cerullo, G., Polli, D., Bregonzio, M. & Otto, C.https://doi.org/10.5281/zenodo.11195903Raman spectroscopic analysis of joint capsule calcification of the fingers (2024)Clinical rheumatology, 43, 1783-1784. Jansen, T. L., Janssen, M., Otto, C., Vosters, J. L. G. & Niessink, T.https://doi.org/10.1007/s10067-024-06941-4Identification of extracellular vesicles from their Raman spectra via self-supervised learning (2024)Scientific reports, 14(1). Article 6791 (E-pub ahead of print/First online). Jensen, M. N., Guerreiro, E. M., Enciso-Martinez, A., Kruglik, S. G., Otto, C., Snir, O., Ricaud, B. & Hellesø, O. G.https://doi.org/10.1038/s41598-024-56788-7The Prevalence of Titanium Dioxide Particles in Synovial Fluid Samples Drops after European Union Ban (2024)Gout, Urate, and Crystal Deposition Disease, 2(1), 45-51. Niessink, T., Janssen, M., Jansen, T. L. & Otto, C.https://doi.org/10.3390/gucdd2010004

2023

Test characteristics of Raman spectroscopy integrated with polarized light microscopy for the diagnosis of acute gouty arthritis (2023)Joint bone spine, 90(6). Article 105611. Niessink, T., Giesen, T., Efdé, M., Comarniceanu, A., Janssen, M., Otto, C. & Jansen, T. L.https://doi.org/10.1016/j.jbspin.2023.105611Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis (2023)Journal of Extracellular Vesicles, 12(11). Article 12376. Lozano-Andrés, E., Enciso-Martinez, A., Gijsbers, A., Ridolfi, A., Van Niel, G., Libregts, S. F. W. M., Pinheiro, C., van Herwijnen, M. J. C., Hendrix, A., Brucale, M., Valle, F., Peters, P. J., Otto, C., Arkesteijn, G. J. A. & Wauben, M. H. M.https://doi.org/10.1002/jev2.12376Clinical image: synovitis of the metacarpophalangeal joints (2023)Annals of the rheumatic diseases, 82, 992-993. Weele, L. v. d., Niessink, T., Maas, M., Griot, P. D., Janssen, M., Otto, C., Jansen, T. L., Tas, S. W. & Vries, N. d.https://doi.org/10.1136/ard-2022-223290Calcium carbonates are novel positive birefringent crystals in synovial fluid (2023)Clinical rheumatology, 42, 635-636. Niessink, T., Otto, C., Janssen, M. & Jansen, T. L.https://doi.org/10.1007/s10067-022-06441-3Raman hyperspectral imaging detects novel and combinations of crystals in synovial fluids of patients with a swollen joint (2023)Journal of raman spectroscopy, 54(1), 47-53. Niessink, T., Kuipers, C., Jong, B. Z. d., Lenferink, A. T. M., Janssen, M., Jansen, T. L. & Otto, C.https://doi.org/10.1002/jrs.6452

2022

Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis (2022)[Working paper › Preprint]. bioRxiv. Lozano-Andrés, E., Enciso-Martinez, A., Gijsbers, A., Libregts, S. F. W. M., Pinheiro, C., Niel, G. V., Hendrix, A., Peters, P. J., Otto, C., Arkesteijn, G. J. A. & Wauben, M. H. M.https://doi.org/10.1101/2022.08.31.506022Clinical Images: Detection of titanium dioxide particles by Raman spectroscopy in synovial fluid from a swollen ankle (2022)Arthritis & rheumatology, 74(6), 1069-1069. Niessink, T., Ringoot, J., Janssen, M., Otto, C. & Jansen, T. L. T. A.https://doi.org/10.1002/art.42078Extracellular Vesicles: A New Source of Biomarkers in Pediatric Solid Tumors? A Systematic Review (2022)Frontiers in oncology, 12. Article 887210. Lak, N. S. M., van der Kooi, E. J., Enciso-Martinez, A., Lozano-Andrés, E., Otto, C., Wauben, M. H. M. & Tytgat, G. A. M.https://doi.org/10.3389/fonc.2022.887210

2021

Fast and non-invasive Raman imaging of material distributions at interfaces (2021)[Thesis › PhD Thesis - Research UT, graduation UT]. University of Twente. Nair, S.https://doi.org/10.3990/1.9789036553124Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy (2021)Analytical chemistry, 93(38), 12966-12972. Nair, S., Gao, J., Otto, C., Duits, M. H. G. & Mugele, F.https://doi.org/10.1021/acs.analchem.1c02581Evaluation of the changes in human milk lipid composition and conformational state with Raman spectroscopy during a breastfeed (2021)Biomedical optics express, 12(7), 3934-3947. de Wolf, J. R., Lenferink, A., Lenferink, A., Otto, C. & Bosschaart, N.https://doi.org/10.1364/BOE.427646Author Correction: Organosilicon uptake by biological membranes (2021)Communications Biology, 4(1). Article 812. Beekman, P., Enciso-Martinez, A., Pujari, S. P., Terstappen, L. W. M. M., Zuilhof, H., Le Gac, S. & Otto, C.https://doi.org/10.1038/s42003-021-02338-0Publisher Correction: Organosilicon uptake by biological membranes (2021)Communications Biology, 4(1). Article 813. Beekman, P., Enciso-Martinez, A., Pujari, S. P., Terstappen, L. W. M. M., Zuilhof, H., Le Gac, S. & Otto, C.https://doi.org/10.1038/s42003-021-02344-2Organosilicon uptake by biological membranes (2021)Communications Biology, 4(1). Article 704. Beekman, P., Enciso-Martinez, A., Pujari, S. P., Terstappen, L. W. M. M., Zuilhof, H. T., Le Gac, S. & Otto, C.https://doi.org/10.1038/s42003-021-02155-5PDMS Curing Inhibition on 3D-Printed Molds: Why? Also, How to Avoid It? (2021)Analytical chemistry, 93(19), 7180-7187. Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Costa, A., Bray, F., Otto, C., Rolando, C. & Le Gac, S.https://doi.org/10.1021/acs.analchem.0c04944In-situ observation of reactive wettability alteration using algorithm-improved confocal Raman microscopy (2021)Journal of colloid and interface science, 584, 551-560. Nair, S., Gao, J., Otto, C., Duits, M. H. G. & Mugele, F.https://doi.org/10.1016/j.jcis.2020.10.016Changes in human milk lipid composition and conformational state during a single breastfeed (2021)In European Conferences on Biomedical Optics, ECBO 2021: Advances in Microscopic Imaging III. Article ETu2A.26 (SPIE Conference Proceedings; Vol. 11922). The Optical Society. de Wolf, J. R., Lenferink, A., Lenferink, A. T. M., Otto, C. & Bosschaart, N.https://doi.org/10.1117/12.2615815

2020

EV trapping: Raman characterization of single tumor-derived extracellular vesicles (2020)[Thesis › PhD Thesis - Research UT, graduation UT]. University of Twente. Enciso Martinez, A.https://doi.org/10.3990/1.9789036550192Cancer-ID: Toward identification of cancer by tumor-derived extracellular vesicles in blood (2020)Frontiers in oncology, 10. Article 608. Rikkert, L. G., Beekman, P., Caro, J., Coumans, F. A. W., Enciso-Martinez, A., Jenster, G., Le Gac, S., Lee, W., van Leeuwen, T. G., Loozen, G. B., Nanou, A., Nieuwland, R., Offerhaus, H. L., Otto, C., Pegtel, D. M., Piontek, M. C., van der Pol, E., de Rond, L., Roos, W. H., … Terstappen, L. W. M. M.https://doi.org/10.3389/fonc.2020.00608

Research profiles

I enjoy teaching

Affiliated study programs

Raman-based diagnostics

Biomolecular distributions in cells

Enzyme activity in cells with Resonance Raman spectroscopy and imaging

Address

University of Twente

Carré (building no. 15), room C4427
Hallenweg 23
7522 NH Enschede
Netherlands

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