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I love thinking that leads to understanding and action.
Expertise Chemistry Procedure Optical Microscopy Biochemistry, Genetics and Molecular Biology Sample Raman Spectroscopy Spectrum Molecule Medicine and Dentistry Organisations Research interest: Development of optical spectroscopy and microscopy techniques for applications in biophysics and biomedics.
A selection of highlights:
first to show Raman spectra of single cells (1990)
first to show Raman images of single cells (2003)
Realization and discovery of "easy CARS" (after A. Voroshilov) that enabled CARS microscopy (1995-1997)
Developed a suite of optical single molecule techniques (imaging, FCS, multicolor FCS, super-resolution localization) for application in biophysics (2000-2010)
Initiative of light-harvesting bacterial protein based biomimicry and associated technology (2002-2010)
Developed Raman integrated with scanning electron microscopy technology (1995-now)
Developed spontaneous and coherent Raman methods (entire career)
Founder of the company Hybriscan Technologies B.V. (2009)
Hybriscan commercialized Raman for SEM, an integrated approach (2011)
Hybriscan commercialized an automated Raman instrument for clinical applications (2022)
Publications Jump to: 2024 | 2023 | 2022 | 2021
Identification of extracellular vesicles from their Raman spectra via self-supervised learning (2024) Scientific reports, 14 (1). Article 6791. Jensen, M. N., Guerreiro, E. M., Enciso-Martinez, A., Kruglik, S. G., Otto, C. , Snir, O., Ricaud, B. & Hellesø, O. G.https://doi.org/10.1038/s41598-024-56788-7 Synovial fluid analysis with compensated polarization light microscopy: a physics approach to quantitative understanding of birefringence, polarization, and Maltese crosses (2024) Clinical rheumatology, 43 (12), 3597-3602. Niessink, T., Jansen, T. L., Kleinherenbrink, M., Janssen, M. & Otto, C. https://doi.org/10.1007/s10067-024-07185-y A new perspective on crystallopathies in rheumatology and orthopaedics with Raman spectroscopy (2024) [Thesis › PhD Thesis - Research UT, graduation UT]. University of Twente. Niessink, T. https://doi.org/10.3990/1.9789036563475 Raman Spectroscopy Reveals Microparticles in Synovial Fluids of Patients With Suspected Implant-Related Complications (2024) Journal of raman spectroscopy (E-pub ahead of print/First online). Niessink, T., Schoenmakers, J. A., Janssen, M., Wouthuyzen-Bakker, M., Piersma, S., van Oosten, M., van Dijl, J., Otto, C. & Jansen, T.https://doi.org/10.1002/jrs.6753 Discovery of calcite as a new pro-inflammatory calcium-containing crystal in human osteoarthritic synovial fluid (2024) Osteoarthritis and cartilage, 32 (10), 1261-1272. Niessink, T., Stassen, R. H. M. J., Kischkel, B., Vuscan, P., Emans, P. J., van den Akker, G. G. H., Janssen, M., Joosten, L. A. B., Otto, C. , Welting, T. J. M. & Jansen, T. L.https://doi.org/10.1016/j.joca.2024.05.004 An objective diagnosis of gout and calcium pyrophosphate deposition disease with machine learning of Raman spectra acquired in a point-of-care setting (2024) Rheumatology (E-pub ahead of print/First online). Niessink, T., Jansen, T. L., Coumans, F. A. W., Welting, T. J. M., Janssen, M. & Otto, C. https://doi.org/10.1093/rheumatology/keae472 Diagnostic Accuracy of Raman Spectroscopy Integrated With Polarized Light Microscopy for Calcium Pyrophosphate–Associated Arthritis (2024) Arthritis care & research, 76 (9), 1333-1341. Niessink, T., Janssen, M., Giesen, T., Efdé, M. N., Comarniceanu, A. C., Otto, C. & Jansen, T. L.https://doi.org/10.1002/acr.25350 High-Resolution Raman Imaging of >300 Patient-Derived Cells from Nine Different Leukemia Subtypes: A Global Clustering Approach (2024) Analytical chemistry, 96 (23), 9468–9477. Vanna, R., Masella, A., Bazzarelli, M., Ronchi, P., Lenferink, A., Tresoldi, C., Morasso, C., Bedoni, M., Cerullo, G., Polli, D., Ciceri, F., De Poli, G., Bregonzio, M. & Otto, C. https://doi.org/10.1021/acs.analchem.4c00787 Supplementary data to "High-Resolution Raman Imaging of >300 Patient-Derived Cells from Nine Different Leukemia Subtypes: A Global Clustering Approach" (2024) [Dataset Types › Dataset]. Zenodo. Vanna, R., Masella, A., Manuela, B., Lenferink, A., Morasso, C., Cerullo, G., Polli, D., Bregonzio, M. & Otto, C. https://doi.org/10.5281/zenodo.11195903 Raman spectroscopic analysis of joint capsule calcification of the fingers (2024) Clinical rheumatology, 43 , 1783-1784. Jansen, T. L., Janssen, M., Otto, C. , Vosters, J. L. G. & Niessink, T.https://doi.org/10.1007/s10067-024-06941-4 The Prevalence of Titanium Dioxide Particles in Synovial Fluid Samples Drops after European Union Ban (2024) Gout, Urate, and Crystal Deposition Disease, 2 (1), 45-51. Niessink, T., Janssen, M., Jansen, T. L. & Otto, C. https://doi.org/10.3390/gucdd2010004 Test characteristics of Raman spectroscopy integrated with polarized light microscopy for the diagnosis of acute gouty arthritis (2023) Joint bone spine, 90 (6). Article 105611. Niessink, T., Giesen, T., Efdé, M., Comarniceanu, A., Janssen, M., Otto, C. & Jansen, T. L.https://doi.org/10.1016/j.jbspin.2023.105611 Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis (2023) Journal of Extracellular Vesicles, 12 (11). Article 12376. Lozano-Andrés, E., Enciso-Martinez, A., Gijsbers, A., Ridolfi, A., Van Niel, G., Libregts, S. F. W. M., Pinheiro, C., van Herwijnen, M. J. C., Hendrix, A., Brucale, M., Valle, F., Peters, P. J., Otto, C. , Arkesteijn, G. J. A. & Wauben, M. H. M.https://doi.org/10.1002/jev2.12376 Clinical image: synovitis of the metacarpophalangeal joints (2023) Annals of the rheumatic diseases, 82 , 992-993. Weele, L. v. d., Niessink, T., Maas, M., Griot, P. D., Janssen, M., Otto, C. , Jansen, T. L., Tas, S. W. & Vries, N. d.https://doi.org/10.1136/ard-2022-223290 Calcium carbonates are novel positive birefringent crystals in synovial fluid (2023) Clinical rheumatology, 42 , 635-636. Niessink, T., Otto, C. , Janssen, M. & Jansen, T. L.https://doi.org/10.1007/s10067-022-06441-3 Raman hyperspectral imaging detects novel and combinations of crystals in synovial fluids of patients with a swollen joint (2023) Journal of raman spectroscopy, 54 (1), 47-53. Niessink, T., Kuipers, C., Jong, B. Z. d., Lenferink, A. T. M., Janssen, M., Jansen, T. L. & Otto, C. https://doi.org/10.1002/jrs.6452 Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis (2022) [Working paper › Preprint]. bioRxiv. Lozano-Andrés, E., Enciso-Martinez, A., Gijsbers, A., Libregts, S. F. W. M., Pinheiro, C., Niel, G. V., Hendrix, A., Peters, P. J., Otto, C. , Arkesteijn, G. J. A. & Wauben, M. H. M. https://doi.org/10.1101/2022.08.31.506022 Clinical Images: Detection of titanium dioxide particles by Raman spectroscopy in synovial fluid from a swollen ankle (2022) Arthritis & rheumatology, 74 (6), 1069-1069. Niessink, T., Ringoot, J., Janssen, M., Otto, C. & Jansen, T. L. T. A.https://doi.org/10.1002/art.42078 Extracellular Vesicles: A New Source of Biomarkers in Pediatric Solid Tumors? A Systematic Review (2022) Frontiers in oncology, 12 . Article 887210. Lak, N. S. M., van der Kooi, E. J., Enciso-Martinez, A., Lozano-Andrés, E., Otto, C. , Wauben, M. H. M. & Tytgat, G. A. M.https://doi.org/10.3389/fonc.2022.887210 Fast and non-invasive Raman imaging of material distributions at interfaces (2021) [Thesis › PhD Thesis - Research UT, graduation UT]. University of Twente. Nair, S. https://doi.org/10.3990/1.9789036553124 Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy (2021) Analytical chemistry, 93 (38), 12966-12972. Nair, S., Gao, J., Otto, C. , Duits, M. H. G. & Mugele, F.https://doi.org/10.1021/acs.analchem.1c02581 Evaluation of the changes in human milk lipid composition and conformational state with Raman spectroscopy during a breastfeed (2021) Biomedical optics express, 12 (7), 3934-3947. de Wolf, J. R., Lenferink, A., Lenferink, A., Otto, C. & Bosschaart, N.https://doi.org/10.1364/BOE.427646 Author Correction: Organosilicon uptake by biological membranes (2021) Communications Biology, 4 (1). Article 812. Beekman, P., Enciso-Martinez, A., Pujari, S. P., Terstappen, L. W. M. M., Zuilhof, H., Le Gac, S. & Otto, C. https://doi.org/10.1038/s42003-021-02338-0 Publisher Correction: Organosilicon uptake by biological membranes (2021) Communications Biology, 4 (1). Article 813. Beekman, P., Enciso-Martinez, A., Pujari, S. P., Terstappen, L. W. M. M., Zuilhof, H., Le Gac, S. & Otto, C. https://doi.org/10.1038/s42003-021-02344-2 Organosilicon uptake by biological membranes (2021) Communications Biology, 4 (1). Article 704. Beekman, P., Enciso-Martinez, A., Pujari, S. P., Terstappen, L. W. M. M., Zuilhof, H. T., Le Gac, S. & Otto, C. https://doi.org/10.1038/s42003-021-02155-5 Research profiles I enjoy teaching
Affiliated study programs Raman-based diagnostics
Biomolecular distributions in cells
Enzyme activity in cells with Resonance Raman spectroscopy and imaging