dr.ir. J.E. van Dongen (Nienke)

Nienke van Dongen received her BSc and MSc in molecular life sciences from Wageningen University, The Netherlands. During her MSc, she specialized in physical chemistry and took part in the top sector chemistry program for talented students in analytical chemistry (TI-COAST).

After graduating in 2018, she joined the BIOS lab on a chip group at the University of Twente to start a PhD project on optical cancer biomarker detection on-chip under the supervision of prof. dr. ir. Segerink and prof dr. Eijkel. From November 2022 onwards she works in the same group as a postdoctoral researcher. Her work focusses mainly on integrating CRISPR/Cas biosensing techniques in microfluidic devices to enable cancer biomarker detection in urine.

Medicine & Life Sciences

# Clustered Regularly Interspaced Short Palindromic Repeats
# Methylation
# Tumor Biomarkers

Physics & Astronomy

# Deoxyribonucleic Acid
# Methylation
# Nuclease

Engineering & Materials Science

# Methylation
# Nucleic Acids

Faculty of Electrical Engineering, Mathematics and Computer Science (EEMCS), Biomedical and Environmental Sensorsystems (BIOS)

My research focuses on (optical) cancer biomarker detection in urine. However, instead of looking at the color or assessing the taste of the urine, the focus is on the biomarkers present in the urine. Biomarkers are biomolecules that, at certain levels, can indicate whether people are ill, what type of disease they suffer from, and what the stage of the disease is.

By enabling cancer biomarker detection, I work on two different approaches, namely Plasmonic Detection which covers gold nanoparticle-based techniques for (digital) DNA molecule detection, and CRISPR/Cas Diagnostics, where we focus on the use of the Cas12a protein for the detection of specific tumor-DNA sequences.

Zhu, P. , Papadimitriou, V. , van Dongen, J. E., Cordeiro, J., Neeleman, Y., Santoso, A., Chen, S. , Eijkel, J. C. T., Peng, H. , Segerink, L., & Rwei, A. (2023). An optical aptasensor for real-time quantification of endotoxin: From ensemble to single-molecule resolution. Science advances, 9(6), Article eadf5509. https://doi.org/10.1126/sciadv.adf5509

van Dongen, J. E. , Berendsen, J. T. W. , Eijkel, J. C. T. , & Segerink, L. (2022). Digital single cancer biomarker detection by CRISPR/Cas sensing: Towards dynamic profiling for CpG methylation quantification. Paper presented at NanoBioTech-Montreux Conference 2022, Montreux, Switzerland.

Dongen, J. E. V. , Eijkel, J. C. T. , & Segerink, L. I. (2022). DNA-Origami Enabled Distance-Dependent Sensing. In 2022 IEEE Sensors IEEE. https://doi.org/10.1109/SENSORS52175.2022.9967092

van Dongen, J. E. (2022). Novel Biosensors for Early Cancer Detection: Development of Gold Nanoparticle and CRISPR/Cas Assays for the Optical Detection of Cancer Biomarkers in Urine. [PhD Thesis - Research UT, graduation UT, University of Twente]. University of Twente. https://doi.org/10.3990/1.9789464586800

Hamacher, T. , Berendsen, J. T. W. , van Dongen, J. E., van der Hee, R. M. , Cornelissen, J. J. L. M., Broekhuijse, M. L. W. J. , & Segerink, L. (2021). Virus removal from semen with a pinched-flow fractionation microfluidic chip. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA (pp. 1193-1194). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). The Chemical and Biological Microsystems Society.

van Dongen, J. E. , Berendsen, J. T. W. , Eijkel, J. C. T. , & Segerink, L. (2021). Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA (pp. 741-742)

van Dongen, J. E. , Spoelstra, L. R. , Berendsen, J. T. W. , Loessberg-Zahl, J. T. , Eijkel, J. C. T. , & Segerink, L. I. (2021). A Multiplexable Plasmonic Hairpin-DNA Sensor Based On Target-specific Tether Dynamics. ACS sensors, 6(12), 4297–4303. https://doi.org/10.1021/acssensors.1c02097

Hamacher, T. , Berendsen, J. T. W. , van Dongen, J. E., van der Hee, R. M. , Cornelissen, J. J. L. M., Broekhuijse, M. L. W. J. , & Segerink, L. I. (2021). Virus removal from semen with a pinched flow fractionation microfluidic chip. Lab on a chip, 21(22), 4477-4486. https://doi.org/10.1039/D1LC00643F

van Dongen, J. E. , Berendsen, J. T. W. , Eijkel, J. C. T. , & Segerink, L. (2021). Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites. Poster session presented at 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States.

van Dongen, J. E. , Berendsen, J. T. W. , Eijkel, J. C. T. , & Segerink, L. I. (2021). A CRISPR/Cas12a-assisted in vitro diagnostic tool for identification and quantification of single CpG methylation sites. Biosensors & bioelectronics, 194, Article 113624. https://doi.org/10.1016/j.bios.2021.113624

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j.e.vandongen@utwente.nl

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University of Twente
Faculty of Electrical Engineering, Mathematics and Computer Science
Carré C2405
P.O. Box 217
7500 AE Enschede
The Netherlands

Faculty of Electrical Engineering, Mathematics and Computer Science (EEMCS), Biomedical and Environmental Sensorsystems (BIOS)

University of Twente Drienerlolaan 5
7522 NB Enschede

+31 (0)53 489 9111
info@utwente.nl
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