dr.ir. J.E. van Dongen | People Pages: Find employees & contact details | University of Twente

Nienke van Dongen received her BSc and MSc in molecular life sciences from Wageningen University, The Netherlands. During her MSc, she specialized in physical chemistry and took part in the top sector chemistry program for talented students in analytical chemistry (TI-COAST).

After graduating in 2018, she joined the BIOS lab on a chip group at the University of Twente to start a PhD project on optical cancer biomarker detection on-chip under the supervision of prof. dr. ir. Segerink and prof dr. Eijkel. From November 2022 onwards she works in the same group as a postdoctoral researcher. Her work focusses mainly on integrating CRISPR/Cas biosensing techniques in microfluidic devices to enable cancer biomarker detection in urine.

Expertise

Chemistry
- Deoxyribonucleic Acid
- Methylation
- Biological Marker
- Procedure
Biochemistry, Genetics and Molecular Biology
- DNA
- Optics
- Molecule
- CRISPR

Organisations

My research focuses on (optical) cancer biomarker detection in urine. However, instead of looking at the color or assessing the taste of the urine, the focus is on the biomarkers present in the urine. Biomarkers are biomolecules that, at certain levels, can indicate whether people are ill, what type of disease they suffer from, and what the stage of the disease is.

By enabling cancer biomarker detection, I work on two different approaches, namely Plasmonic Detection which covers gold nanoparticle-based techniques for (digital) DNA molecule detection, and CRISPR/Cas Diagnostics, where we focus on the use of the Cas12a protein for the detection of specific tumor-DNA sequences.

Publications

2023

An optical aptasensor for real-time quantification of endotoxin: From ensemble to single-molecule resolution, Article eadf5509. Zhu, P., Papadimitriou, V., van Dongen, J. E., Cordeiro, J., Neeleman, Y., Santoso, A., Chen, S., Eijkel, J. C. T., Peng, H., Segerink, L. & Rwei, A.https://doi.org/10.1126/sciadv.adf5509

2022

DNA-Origami Enabled Distance-Dependent SensingIn 2022 IEEE Sensors. IEEE. Dongen, J. E. V., Eijkel, J. C. T. & Segerink, L. I.https://doi.org/10.1109/SENSORS52175.2022.9967092Digital single cancer biomarker detection by CRISPR/Cas sensing: Towards dynamic profiling for CpG methylation quantification. van Dongen, J. E., Berendsen, J. T. W., Eijkel, J. C. T. & Segerink, L.Novel Biosensors for Early Cancer Detection: Development of Gold Nanoparticle and CRISPR/Cas Assays for the Optical Detection of Cancer Biomarkers in Urine. University of Twente. van Dongen, J. E.https://doi.org/10.3990/1.9789464586800

2021

A Multiplexable Plasmonic Hairpin-DNA Sensor Based On Target-specific Tether Dynamics, 4297–4303. van Dongen, J. E., Spoelstra, L. R., Berendsen, J. T. W., Loessberg-Zahl, J. T., Eijkel, J. C. T. & Segerink, L. I.https://doi.org/10.1021/acssensors.1c02097A CRISPR/Cas12a-assisted in vitro diagnostic tool for identification and quantification of single CpG methylation sites, Article 113624. van Dongen, J. E., Berendsen, J. T. W., Eijkel, J. C. T. & Segerink, L. I.https://doi.org/10.1016/j.bios.2021.113624Virus removal from semen with a pinched flow fractionation microfluidic chip, 4477-4486. Hamacher, T., Berendsen, J. T. W., van Dongen, J. E., van der Hee, R. M., Cornelissen, J. J. L. M., Broekhuijse, M. L. W. J. & Segerink, L. I.https://doi.org/10.1039/D1LC00643FTowards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites. van Dongen, J. E., Berendsen, J. T. W., Eijkel, J. C. T. & Segerink, L.Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites.In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA (pp. 741-742). van Dongen, J. E., Berendsen, J. T. W., Eijkel, J. C. T. & Segerink, L.Virus removal from semen with a pinched-flow fractionation microfluidic chipIn MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA (pp. 1193-1194). The Chemical and Biological Microsystems Society. Hamacher, T., Berendsen, J. T. W., van Dongen, J. E., van der Hee, R. M., Cornelissen, J. J. L. M., Broekhuijse, M. L. W. J. & Segerink, L.

Research profiles

Address

University of Twente

Carré (building no. 15), room C2405
Hallenweg 23
7522 NH Enschede
Netherlands

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Organisations