Nienke van Dongen received her BSc and MSc in molecular life sciences from Wageningen University, The Netherlands. During her MSc, she specialized in physical chemistry and took part in the top sector chemistry program for talented students in analytical chemistry (TI-COAST). 

After graduating in 2018, she joined the BIOS lab on a chip group at the University of Twente to start a PhD project on optical cancer biomarker detection on-chip under the supervision of prof. dr. ir. Segerink and prof dr. Eijkel. From November 2022 onwards she works in the same group as a postdoctoral researcher. Her work focusses mainly on integrating CRISPR/Cas biosensing techniques in microfluidic devices to enable cancer biomarker detection in urine. 

Expertise

  • Chemistry

    • Deoxyribonucleic Acid
    • Methylation
    • Biological Marker
    • Procedure
  • Biochemistry, Genetics and Molecular Biology

    • DNA
    • Optics
    • Molecule
    • CRISPR

Organisations

My research focuses on (optical) cancer biomarker detection in urine. However, instead of looking at the color or assessing the taste of the urine, the focus is on the biomarkers present in the urine. Biomarkers are biomolecules that, at certain levels, can indicate whether people are ill, what type of disease they suffer from, and what the stage of the disease is. 

By enabling cancer biomarker detection, I work on two different approaches, namely Plasmonic Detection which covers gold nanoparticle-based techniques for (digital) DNA molecule detection, and CRISPR/Cas Diagnostics, where we focus on the use of the Cas12a protein for the detection of specific tumor-DNA sequences. 

Publications

2023

Digital Single Cancer Biomarker Detection Using CRISPR/Cas Sensing (2023)[Contribution to conference › Poster] CRISPR 2023. van Dongen, J. E., Berendsen, J. T. W., Eijkel, J. C. T. & Segerink, L.An optical aptasensor for real-time quantification of endotoxin: From ensemble to single-molecule resolution (2023)Science advances, 9(6). Article eadf5509. Zhu, P., Papadimitriou, V., van Dongen, J. E., Cordeiro, J., Neeleman, Y., Santoso, A., Chen, S., Eijkel, J. C. T., Peng, H., Segerink, L. & Rwei, A.https://doi.org/10.1126/sciadv.adf5509

2021

A Multiplexable Plasmonic Hairpin-DNA Sensor Based On Target-specific Tether Dynamics (2021)ACS sensors, 6(12), 4297–4303. van Dongen, J. E., Spoelstra, L. R., Berendsen, J. T. W., Loessberg-Zahl, J. T., Eijkel, J. C. T. & Segerink, L. I.https://doi.org/10.1021/acssensors.1c02097A CRISPR/Cas12a-assisted in vitro diagnostic tool for identification and quantification of single CpG methylation sites (2021)Biosensors & bioelectronics, 194. Article 113624. van Dongen, J. E., Berendsen, J. T. W., Eijkel, J. C. T. & Segerink, L. I.https://doi.org/10.1016/j.bios.2021.113624Virus removal from semen with a pinched flow fractionation microfluidic chip (2021)Lab on a chip, 21(22), 4477-4486. Hamacher, T., Berendsen, J. T. W., van Dongen, J. E., van der Hee, R. M., Cornelissen, J. J. L. M., Broekhuijse, M. L. W. J. & Segerink, L. I.https://doi.org/10.1039/D1LC00643F

Research profiles

Courses academic year 2023/2024

Courses in the current academic year are added at the moment they are finalised in the Osiris system. Therefore it is possible that the list is not yet complete for the whole academic year.

Address

University of Twente

Carré (building no. 15), room C2405
Hallenweg 23
7522 NH Enschede
Netherlands

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