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dr. K. Govindaraj MSc (Kannan)

Researcher

About Me

I'm a molecular cell biologist, currently (May, 2019 - present) investigating the effect of macromolecular crowding in health and disease. In my doctoral research (2014 - 2019), I developed a tool "Transcription Factor - Fluorescence Recovery After Photobleaching (TF-FRAP)" to quantify transcription factor dynamics and activity. I also worked as a Junior Research Fellow at the Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India. Where, I investigated trailing edge vesicles in osteoblast cells. I hold a master's degree in Molecular Biosciences from Bishop Heber College (Bharathidasan University), Trichy, India.

Expertise

Medicine & Life Sciences
Fluorescence Recovery After Photobleaching
Sox9 Transcription Factor
Transcription Factors
Engineering & Materials Science
Biocompatibility
Glucose
Osteoblasts
Polyethylene Oxides
Chemistry
Glycopolymer

Research

Osteoarthritis (OA) is a cartilage degenerative disease and millions are suffering worldwide. Cartilage degeneration is the result of imbalance between anabolic and catabolic processes. In addition to that, increased expression of proteolytic enzymes such as matrix metalloproteinases and aggrecanases also play an important role in the onset of the disease. Treating OA is limited to symptomatic treatments as drugs curing OA is not yet developed as our current understanding about molecular mechanisms involved in this disease progression is not yet fully explored.

Understanding the switching mechanism between hypertrophic cartilage and permanent cartilage will give new insights in to therapeutic possibilities in the cartilage disease. SOX9 and RUNX2 are the master transcription factors for the cartilage and bone development respectively and and their dysfunction is often detected in osteoarthritis (OA). My reasearch is focus on the differential regulation of these transcription factors during chondrocyte development, homeostasis and cartilage disease. To study their activity, we apply biophysical techniques, such as Transcription Factor - Fluorescence Recovery After Photobleaching (TF-FRAP), and measure the dynamics of these transcription factors in human primary chondrocytes and mesenchymal stem cells. To further understand the role of SOX9 in OA pathophysiology, we study their activity in response to extracellular stimuli. 

Publications

Recent
Govindaraj, K., Meteling, M., van Rooij, J., Becker, M., van Wijnen, A. J., van den Beucken, J. J. J. P., Ramos, Y. F. M., van Meurs, J. , Post, J. N. , & Leijten, J. (2024). Osmolarity-Induced Altered Intracellular Molecular Crowding Drives Osteoarthritis Pathology. Advanced science, Article 2306722. Advance online publication. https://doi.org/10.1002/advs.202306722
Govindaraj, K., Meteling, M. A. W. , & Leijten, J. (2023). Altered intracellular molecular crowding plays a key role in osteoarthritis pathology. Poster session presented at 47th FEBS Congress 2023, Tours, France.
Allawadhi, P., Singh, V. , Govindaraj, K., Khurana, I., Sarode, L. P., Navik, U., Banothu, A. K., Weiskirchen, R., Bharani, K. K. , & Khurana, A. (2022). Biomedical applications of polysaccharide nanoparticles for chronic inflammatory disorders: Focus on rheumatoid arthritis, diabetes and organ fibrosis. Carbohydrate polymers, 281, Article 118923. https://doi.org/10.1016/j.carbpol.2021.118923
Govindaraj, K. , & Post, J. N. (2021). Using FRAP to quantify changes in transcription factor dynamics after cell stimulation: Cell culture, FRAP, data analysis, and visualization. In A. J. van Wijnen, & M. S. Ganshina (Eds.), Osteoporosis and Osteoarthritis (pp. 109-139). (Methods in Molecular Biology; Vol. 2221). Humana Press. https://doi.org/10.1007/978-1-0716-0989-7_9
Govindaraj, K. (2020). Quantifying Transcriptional Dynamics by TF-FRAP. [PhD Thesis - Research UT, graduation UT, University of Twente]. University of Twente. https://doi.org/10.3990/1.9789036550765

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Contact Details

Visiting Address

University of Twente
Faculty of Science and Technology
Horst Complex (building no. 20), room ZH147
De Horst 2
7522LW  Enschede
The Netherlands

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Mailing Address

University of Twente
Faculty of Science and Technology
Horst Complex  ZH147
P.O. Box 217
7500 AE Enschede
The Netherlands

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