TNW-BET-AST
Workdays
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odd
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on campus
work from home
out of office

Expertise

  • Biochemistry, Genetics and Molecular Biology

    • Embryoid Body
    • Induced Pluripotent Stem Cell
  • Chemistry

    • Polycarbonate
    • Pore Size
  • Medicine and Dentistry

    • Aging
    • Culture
    • Organoid
    • Tissues

Organisations

The brain is connected to the gastrointestinal tract through bi-directional biochemical signaling pathways which are largely regulated by the composition of the microbiome. My goal is to develop a gut-brain axis on a chip (GBOC) to enable to study the complex interplay between the gut microbiome and brain health. The envisaged microfluidic controlled chip set-up recapitulates the two major barriers separating the brain and the gastrointestinal tract: the gut-blood barrier and the blood-brain barrier making use of human pluripotent stem cell-derived technology. The strength of the developed GBOC technology will be demonstrated with a proof-of-concept model of the impact of microbiota variation on neuronal functioning.

This project is part of the Netherlands Organ-on-a-chip Initiative - NOCI.

https://noci-organ-on-chip.nl/

Publications

2024

The Gut-Brain Axis on Chip to unravel Inter-Tissue Interactions (2024)[Thesis › PhD Thesis - Research UT, graduation UT]. University of Twente. Koch, L. S.https://doi.org/10.3990/1.9789036563253Standardizing designed and emergent quantitative features in microphysiological systems (2024)Nature Biomedical Engineering, 8, 941-962. Nahon, D., Moerkens, R., Aydogmus, H., Lendemeijer, B., Martínez-Silgado, A., Stein, J., Dostanić, M., Frimat, J.-P., Gontan, C., de Graaf, M. N. S., Hu, M., Kasi, D., Koch, L. S., Le, K., Lim, S., Middelkamp, H. H. T., Mooiweer, J., Montreux-Ragot, P., Niggl, E., … Mummery, C. L.https://doi.org/10.1038/s41551-024-01236-0

2023

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research (2023)Journal of visualized experiments, 2023(195). Article e64586. Koch, L. S., Buentello, D. C. & Broersen, K.https://doi.org/10.3791/64586

2022

Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies (2022)PLoS ONE, 17(5 May). Article e0262062. Buentello, D. C., Koch, L. S., Trujillo-De Santiago, G., Alvarez, M. M. & Broersen, K.https://doi.org/10.1371/journal.pone.0262062

2021

Transwell-Integrated 2 µm Thick Transparent Polydimethylsiloxane Membranes with Controlled Pore Sizes and Distribution to Model the Blood-Brain Barrier (2021)Advanced Materials Technologies, 6(12). Article 2100138. Zakharova, M., Tibbe, M. P., Koch, L. S., Le-The, H., Leferink, A. M., van den Berg, A., van der Meer, A. D., Broersen, K. & Segerink, L. I.https://doi.org/10.1002/admt.202100138

Other contributions

Choy Buentello, D., Koch, L. S., Trujillo-de Santiago, G., Alvarez, M. M., & Broersen, K. (2022). Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies. Plos one, 17(5), e0262062.

Research profiles

Address

University of Twente

Horst Complex (building no. 20), room ZH125
De Horst 2
7522 LW Enschede
Netherlands

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