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M. Zakharova MSc (Mariia)

PhD Candidate

Expertise

Engineering & Materials Science
Astrocytes
Blood-Brain Barrier
Cell Culture
Membranes
Polydimethylsiloxane
Pore Size
Tissue
Medicine & Life Sciences
Blood-Brain Barrier

Research

Multiplexed Organ on a Chip

The main limitation of the current drug testing is the lack of appropriate models that adequately mimic the human environment. Conventional cell culturing and animal models are not suitable for correct drug testing being either too simple or not physiologically relevant to the human organism. Therefore, an organ-on-a-chip (OOC) system has gained a significant interest among the scientist as an alternative to the conventional studies of the human organs and its reaction on different stimuli (e.g. drugs). However, one of the significant disadvantages of existing OOC models is their low throughput, thus, it is not possible to test different experimental stimuli in parallel, limiting readout possibilities.

The aim of this research is to develop a parallelized, standardized and automated model suitable for multiplexing of organ-on-a-chip systems with further integrated sensors for simultaneous, real-time analysis of the effect of different stimuli on the barrier function. This high-throughput screening platform may enhance the drug screening process and help to minimize the animal experiments.

Publications

Recent
Zakharova, M., Persijn van Meerten, P. A. M. , van den Broek, M. R. P. , Segerink, L. , & Segers, T. J. (2021). Transient disruption of a Blood-brain Barrier on-chip using focused ultrasound and monodisperse microbubbles. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA (pp. 477478)
Paggi, C. A. , Zakharova, M. , Segerink, L. , le Gac, S. , Moreira Teixeira Leijten, L. S. , & Karperien, M. (2020). Synovial membrane on chip: a new tool to study co-culture response to mechanical stimulation. In Proceedings of the 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 [Th8-854.e]
Bossink, E. G. B. M. , Zakharova, M. , Odijk, M. , & Segerink, L. (2020). Measuring barrier function in a gut-on-chip. In μTAS 2020: The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 943-944)
Zakharova, M. , van der Helm, M., do Carmo, M. P. , Le-The, H. , Tibbe, M. , van der Meer, A. , Broersen, K. , Eijkel, J. , & Segerink, L. (2019). Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 432-433). The Chemical and Biological Microsystems Society.
Zakharova, M. , van der Helm, M., Palma Do Carmo, M. , Le The, H. , Tibbe, M. , van der Meer, A. , Broersen, K. , Eijkel, J. , & Segerink, L. (2019). Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function. Poster session presented at 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, Basel, Switzerland.

Contact Details

Visiting Address

University of Twente
Drienerlolaan 5
7522 NB Enschede
The Netherlands

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Mailing Address

University of Twente
P.O. Box 217
7500 AE Enschede
The Netherlands